ArticlePlant physiology2006
Cloning and expression analysis of a UDP-galactose/glucose pyrophosphorylase from melon fruit provides evidence for the major metabolic pathway of galactose metabolism in raffinose oligosaccharide metabolizing plants.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed.
- Genetic control of tomato fruit cracking through SlPMEI27-dependent pectin modification.Molecular horticulture · 2026Article
- Characterization, Expression Profile Analysis, and Functional Prediction ofPlants (Basel, Switzerland) · 2025Article
- Insights into Sucrose Metabolism and Its Ethylene-Dependent Regulation in Cucumis melo L.Molecular biotechnology · 2025Review
- Restoring galactose metabolism without restoring GALT rescues both compromised survival in larvae and an adult climbing deficit in a GALT-null D. Melanogaster model of classic galactosemia.Journal of inherited metabolic disease · 2024Article
- Article
- Unraveling the genetic basis of oil quality in olives: a comparative transcriptome analysis.Frontiers in plant science · 2024Article
- Phloem unloading in cultivated melon fruits follows an apoplasmic pathway during enlargement and ripening.Horticulture research · 2023Article
- Untargeted Metabolomics Coupled with Chemometrics for Leaves and Stem Barks of DioeciousMetabolites · 2022Article
- Identification of Key Gene Networks Controlling Soluble Sugar and Organic Acid Metabolism During Oriental Melon Fruit Development by Integrated Analysis of Metabolic and Transcriptomic Analyses.Frontiers in plant science · 2022Article
- Comparative Analysis of the Transcriptomes of Persisting and Abscised Fruitlets: Insights into Plant Hormone and Carbohydrate Metabolism Regulated Self-Thinning of Pecan Fruitlets during the Early Stage.Current issues in molecular biology · 2021Article
- Physiological and Proteomic Analyses of Two Acanthus Species to Tidal Flooding Stress.International journal of molecular sciences · 2021Article
- Comparative transcriptome profiling reveals cold stress responsiveness in two contrasting Chinese jujube cultivars.BMC plant biology · 2020Article
- Transcriptome profiling of non-climacteric 'yellow' melon during ripening: insights on sugar metabolism.BMC genomics · 2020Article
- Phosphoglucomutase Is Not the Target for Galactose Toxicity in Plants.Frontiers in plant science · 2020Article
- Overexpression of the tonoplast sugar transporter CmTST2 in melon fruit increases sugar accumulation.Journal of experimental botany · 2018Article
- Metagenomic and Metatranscriptomic Analyses of Diverse Watermelon Cultivars Reveal the Role of Fruit Associated Microbiome in Carbohydrate Metabolism and Ripening of Mature Fruits.Frontiers in plant science · 2018Article
- UDP-Sugar Producing Pyrophosphorylases: Distinct and Essential Enzymes With Overlapping Substrate Specificities, ProvidingFrontiers in plant science · 2018Review
- Sugar metabolism reprogramming in a non-climacteric bud mutant of a climacteric plum fruit during development on the tree.Journal of experimental botany · 2017Article
- Unraveling the complexity of transcriptomic, metabolomic and quality environmental response of tomato fruit.BMC plant biology · 2017Article
- Comparative transcriptome analysis of two contrasting watermelon genotypes during fruit development and ripening.BMC genomics · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Cucurbitaceae translocate a significant portion of their photosynthate as raffinose and stachyose, which are galactosyl derivatives of sucrose. These are initially hydrolyzed by alpha-galactosidase to yield free galactose (Gal) and, accordingly, Gal metabolism is an important pathway in Cucurbitaceae sink tissue. We report here on a novel plant-specific enzyme responsible for the nucleotide activation of phosphorylated Gal and the subsequent entry of Gal into sink metabolism. The enzyme was antibody purified, sequenced, and the gene cloned and functionally expressed in Escherichia coli. The heterologous protein showed the characteristics of a dual substrate UDP-hexose pyrophosphorylase (PPase) with activity toward both Gal-1-P and glucose (Glc)-1-P in the uridinylation direction and their respective UDP-sugars in the reverse direction. The two other enzymes involved in Glc-P and Gal-P uridinylation are UDP-Glc PPase and uridyltransferase, and these were also cloned, heterologously expressed, and characterized. The gene expression and enzyme activities of all three enzymes in melon (Cucumis melo) fruit were measured. The UDP-Glc PPase was expressed in melon fruit to a similar extent as the novel enzyme, but the expressed protein was specific for Glc-1-P in the UDP-Glc synthesis direction and did not catalyze the nucleotide activation of Gal-1-P. The uridyltransferase gene was only weakly expressed in melon fruit, and activity was not observed in crude extracts. The results indicate that this novel enzyme carries out both the synthesis of UDP-Gal from Gal-1-P as well as the subsequent synthesis of Glc-1-P from the epimerase product, UDP-Glc, and thus plays a key role in melon fruit sink metabolism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.