ArticleFEBS letters2006
The non-structural protein Nsp10 of mouse hepatitis virus binds zinc ions and nucleic acids.
Article in FEBS letters, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- Crystal structure of SARS-CoV-2 nsp10-nsp16 in complex with small molecule inhibitors, SS148 and WZ16.Protein science : a publication of the Protein Society · 2022Article
- Expression and immunogenicity of nsp10 protein of porcine epidemic diarrhea virus.Research in veterinary science · 2022Article
- Identification and characterization of alternative sites and molecular probes for SARS-CoV-2 target proteins.Frontiers in chemistry · 2022Article
- Nature's therapy for COVID-19: Targeting the vital non-structural proteins (NSP) from SARS-CoV-2 with phytochemicals from Indian medicinal plants.Phytomedicine plus : international journal of phytotherapy and phytopharmacology · 2021Article
- Functional and druggability analysis of the SARS-CoV-2 proteome.European journal of pharmacology · 2021Article
- Structural and functional insights into non-structural proteins of coronaviruses.Microbial pathogenesis · 2021Review
- High-resolution structures of the SARS-CoV-2 2'-Science signaling · 2020Article
- A Structural View of SARS-CoV-2 RNA Replication Machinery: RNA Synthesis, Proofreading and Final Capping.Cells · 2020Review
- The crystal structure of nsp10-nsp16 heterodimer from SARS-CoV-2 in complex with S-adenosylmethionine.bioRxiv : the preprint server for biology · 2020Article
- The Nonstructural Proteins Directing Coronavirus RNA Synthesis and Processing.Advances in virus research · 2016Review
- SARS-CoV ORF1b-encoded nonstructural proteins 12-16: replicative enzymes as antiviral targets.Antiviral research · 2014Review
- Biochemical and structural insights into the mechanisms of SARS coronavirus RNA ribose 2'-O-methylation by nsp16/nsp10 protein complex.PLoS pathogens · 2011Article
- Molecular mapping of the RNA Cap 2'-O-methyltransferase activation interface between severe acute respiratory syndrome coronavirus nsp10 and nsp16.The Journal of biological chemistry · 2010Article
- Dynamics of coronavirus replication-transcription complexes.Journal of virology · 2010Article
- Bovine coronavirus nonstructural protein 1 (p28) is an RNA binding protein that binds terminal genomic cis-replication elements.Journal of virology · 2009Article
- Crystal structures of the X-domains of a Group-1 and a Group-3 coronavirus reveal that ADP-ribose-binding may not be a conserved property.Protein science : a publication of the Protein Society · 2009Article
- Topology and membrane anchoring of the coronavirus replication complex: not all hydrophobic domains of nsp3 and nsp6 are membrane spanning.Journal of virology · 2008Article
- Variable oligomerization modes in coronavirus non-structural protein 9.Journal of molecular biology · 2008Article
- Complete nucleotide sequence of polyprotein gene 1 and genome organization of turkey coronavirus.Virus research · 2008Article
- The SARS-Coronavirus PLnc domain of nsp3 as a replication/transcription scaffolding protein.Virus research · 2008Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The non-structural protein Nsp10 of coronaviruses is a small cleavage product of the viral replicase polyprotein that has been implicated in RNA synthesis. Nsp10 of mouse hepatitis virus (MHV) displays an apparent molecular mass of 13-16kDa in reducing SDS-PAGE and analytical gel filtration, while dynamic light scattering suggests the existence of oligomeric forms. Atomic absorption spectroscopy reveals two metal ions per Nsp10 monomer, with a preference for Zn(2+) over Fe(2+/3+) and Co(2+). These are probably bound by two Zn-finger-like motifs. Moreover, MHV Nsp10 interacts with tRNA, single-stranded RNA, double-stranded DNA and, to a lesser extent, single-stranded DNA as shown by gel-shift experiments. The K(d) for tRNA is 2.1+/-0.2 microM.
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