ArticleTransgenic research2006
High level expression of tissue-nonspecific alkaline phosphatase in the milk of transgenic rabbits.
Article in Transgenic research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
- Monitoring of Venus transgenic cell migration during pregnancy in non-transgenic rabbits.Transgenic research · 2017Article
- Secretion of a recombinant protein without a signal peptide by the exocrine glands of transgenic rabbits.PloS one · 2017Article
- A novel transgenic rabbit model with reduced repolarization reserve: long QT syndrome caused by a dominant-negative mutation of the KCNE1 gene.British journal of pharmacology · 2016Article
- Transposon-Based Reporter Marking Provides Functional Evidence for Intercellular Bridges in the Male Germline of Rabbits.PloS one · 2016Article
- Transposon-mediated transgenesis, transgenic rescue, and tissue-specific gene expression in rodents and rabbits.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Expression systems and species used for transgenic animal bioreactors.BioMed research international · 2013Article
- Article
- Transgenic rabbit production with simian immunodeficiency virus-derived lentiviral vector.Transgenic research · 2010Article
- Production of pharmaceutical proteins by transgenic animals.Comparative immunology, microbiology and infectious diseases · 2009Review
- Lactation performance of transgenic goats expressing recombinant human butyryl-cholinesterase in the milk.Transgenic research · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alkaline phosphatase is a promising therapeutic agent in the Gram-negative bacterial lipopolysaccharide (LPS) mediated acute and chronic diseases. Contrary to other alkaline phosphatase isozymes, purified tissue-nonspecific alkaline phosphatase (TNAP) is not available in large quantities from tissue sources, which would enable to analyse its efficacy in animal sepsis models. Two transgenic rabbit lines were created by pronuclear microinjection with the whey acidic protein promoter-humanTNAP minigene (WAP-hTNAP). Lactating females of both lines produced biologically active human TNAP. As indicated by fractionation of milk samples the recombinant alkaline phosphatase was associated with the membrane of milk fat globules. Alkaline phosphatase enzymatic activity was two orders of magnitude higher compared to normal human serum levels. The demonstration that this TNAP is physiologically active would provide the clue to use transgenic animals as bioreactor for bulk production of the human tissue-nonspecific alkaline phosphatase in milk. This may be a valuable and possibly viable option with important implication in attenuating LPS mediated inflammatory responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.