ArticleEMBO reports2006
Structural mimicry for vinculin activation by IpaA, a virulence factor of Shigella flexneri.
Article in EMBO reports, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Host-microbiome protein-protein interactions capture disease-relevant pathways.Genome biology · 2022Article
- Manipulation of Focal Adhesion Signaling by Pathogenic Microbes.International journal of molecular sciences · 2021Review
- Using proteomics to identify host cell interaction partners for VgrG and IglJ.Scientific reports · 2020Article
- ELM-the eukaryotic linear motif resource in 2020.Nucleic acids research · 2020Article
- Reaction of antibodies toWorld journal of gastroenterology · 2019Article
- A NovelmSphere · 2018Article
- Integration of actin dynamics and cell adhesion by a three-dimensional, mechanosensitive molecular clutch.Nature cell biology · 2015Review
- Vinculin Interacts with the Chlamydia Effector TarP Via a Tripartite Vinculin Binding Domain to Mediate Actin Recruitment and Assembly at the Plasma Membrane.Frontiers in cellular and infection microbiology · 2015Article
- Identification of an actin binding surface on vinculin that mediates mechanical cell and focal adhesion properties.Structure (London, England : 1993) · 2014Article
- Potential of known and short prokaryotic protein motifs as a basis for novel peptide-based antibacterial therapeutics: a computational survey.Frontiers in microbiology · 2014Article
- Integrin-mediated internalization of Staphylococcus aureus does not require vinculin.BMC cell biology · 2013Article
- αE-catenin is an autoinhibited molecule that coactivates vinculin.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Vinculin activators target integrins from within the cell to increase melanoma sensitivity to chemotherapy.Molecular cancer research : MCR · 2011Article
- New insights into vinculin function and regulation.International review of cell and molecular biology · 2011Review
- Molecular pathogenesis of Shigella spp.: controlling host cell signaling, invasion, and death by type III secretion.Clinical microbiology reviews · 2008Review
- Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD.The Journal of biological chemistry · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Invasion of epithelial cells by Shigella flexneri is characterized by cytoskeletal rearrangements of the host cell membrane, promoting internalization of the bacterium. The bacterial effector IpaA is injected into the epithelial cell by a type III secretion apparatus and recruits vinculin to regulate actin polymerization at the site of entry. We analysed the complex formed between a carboxy-terminal fragment of IpaA (IpaA(560-633)) and the vinculin D1 domain (VD1), both in crystals and in solution. We present evidence that IpaA(560-633) has two alpha-helical vinculin-binding sites that simultaneously bind two VD1 molecules. The interaction of IpaA(560-633) with VD1 is highly similar to the interaction of the endogenous, eukaryotic proteins talin and alpha-actinin with VD1, showing that Shigella uses a structural mimicry strategy to activate vinculin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.