ArticleThe Journal of molecular diagnostics : JMD2006
A quantitative reverse transcriptase-polymerase chain reaction assay to identify metastatic carcinoma tissue of origin.
Article in The Journal of molecular diagnostics : JMD, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 105 citations in OpenAlex.
- The Quest for Improving Treatment of Cancer of Unknown Primary (CUP) Through Molecularly-Driven Treatments: A Systematic Review.Frontiers in oncology · 2020Pooled it
- Review
- 90-Gene Expression Profiling for Tissue Origin Diagnosis of Cancer of Unknown Primary.Frontiers in oncology · 2021Article
- Data independent acquisition-mass spectrometry (DIA-MS)-based comprehensive profiling of bone metastatic cancers revealed molecular fingerprints to assist clinical classifications for bone metastasis of unknown primary (BMUP).Translational cancer research · 2020Article
- Cancer of Unknown Primary Site: Real Entity or Misdiagnosed Disease?Journal of Cancer · 2020Review
- A Novel XGBoost Method to Identify Cancer Tissue-of-Origin Based on Copy Number Variations.Frontiers in genetics · 2020Article
- Carcinoma of Unknown Primary withThe oncologist · 2019Article
- 2018 consensus statement by the Spanish Society of Pathology and the Spanish Society of Medical Oncology on the diagnosis and treatment of cancer of unknown primary.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2018Review
- The Potential Clinical and Economic Value of Primary Tumour Identification in Metastatic Cancer of Unknown Primary Tumour: A Population-Based Retrospective Matched Cohort Study.PharmacoEconomics - open · 2018Article
- Prognostic impact of presumed breast or ovarian cancer among patients with unfavorable-subset cancer of unknown primary site.BMC cancer · 2018Article
- Molecular Diagnostics in Clinical Oncology.Frontiers in molecular biosciences · 2018Review
- Cost-effectiveness of using a gene expression profiling test to aid in identifying the primary tumour in patients with cancer of unknown primary.The pharmacogenomics journal · 2017Article
- Pan-cancer transcriptome analysis reveals a gene expression signature for the identification of tumor tissue origin.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2016Article
- Cancers of unknown primary origin (CUP) are characterized by chromosomal instability (CIN) compared to metastasis of know origin.BMC cancer · 2015Article
- Development and validation of a microRNA based diagnostic assay for primary tumor site classification of liver core biopsies.Molecular oncology · 2015Article
- RNA-Seq accurately identifies cancer biomarker signatures to distinguish tissue of origin.Neoplasia (New York, N.Y.) · 2014Article
- Gene expression profiling in patients with carcinoma of unknown primary site: from translational research to standard of care.Virchows Archiv : an international journal of pathology · 2014Review
- Overview of various techniques/platforms with critical evaluation of each.Current treatment options in oncology · 2013Review
- Papillary Renal Carcinoma Presenting as a Cancer of Unknown Primary (CUP) and Diagnosed through Gene Expression Profiling.Case reports in oncology · 2012Article
- Cancers of unknown primary origin: current perspectives and future therapeutic strategies.Journal of translational medicine · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Identifying the primary site in patients with metastatic carcinoma of unknown primary origin can enable more specific therapeutic regimens and may prolong survival. Twenty-three putative tissue-specific markers for lung, colon, pancreatic, breast, prostate, and ovarian carcinomas were nominated by querying a gene expression profile database and by performing a literature search. Ten of these marker candidates were then selected based on validation by reverse transcriptase-polymerase chain reaction (RT-PCR) on 205 formalin-fixed, paraffin-embedded metastatic carcinoma specimens originating from these six and from other cancer types. Next, we optimized the RNA isolation and quantitative RT-PCR methods for these 10 markers and applied the quantitative RT-PCR assay to a set of 260 metastatic tumors. We then built a gene-based algorithm that predicted the tissue of origin of metastatic carcinomas with an overall leave-one-out cross-validation accuracy of 78%. Lastly, our assay demonstrated an accuracy of 76% when tested on an independent set of 48 metastatic samples, 37 of which were either a known primary or initially presented as carcinoma of unknown primary but were subsequently resolved.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.