ArticleJournal of clinical microbiology2006
Progression toward an improved DNA amplification-based typing technique in the study of Mycobacterium tuberculosis epidemiology.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 2 of them syntheses that pooled it.
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Who cites it
16 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Mycobacterium tuberculosis mixed infections and drug resistance in sub-Saharan Africa: a systematic review.African health sciences · 2022Pooled it
- Pooled it
- Molecular typing of Mycobacterium tuberculosis: a review of current methods, databases, softwares, and analytical tools.FEMS microbiology reviews · 2025Review
- MIRU-VNTR Genotyping of Mycobacterium tuberculosis Strains Using QIAxcel Technology: A Multicentre Evaluation Study.PloS one · 2016Article
- First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains.Journal of clinical microbiology · 2012Article
- Human Mycobacterium bovis infections in London and Southeast England.Journal of clinical microbiology · 2012Article
- Comparison of an automated repetitive sequence-based PCR microbial typing system with IS6110-restriction fragment length polymorphism for epidemiologic investigation of clinical Mycobacterium tuberculosis isolates in Korea.The Korean journal of laboratory medicine · 2011Article
- Application of single-nucleotide polymorphism and mycobacterial interspersed repetitive units-variable number of tandem repeats analyses to clinical Mycobacterium tuberculosis isolates from Korea.The Korean journal of laboratory medicine · 2011Article
- Article
- Associations between Mycobacterium tuberculosis strains and phenotypes.Emerging infectious diseases · 2010Article
- Discriminatory ability of hypervariable variable number tandem repeat loci in population-based analysis of Mycobacterium tuberculosis strains, London, UK.Emerging infectious diseases · 2009Article
- Prospective universal application of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping to characterize Mycobacterium tuberculosis isolates for fast identification of clustered and orphan cases.Journal of clinical microbiology · 2009Article
- DNA restriction fragment length polymorphism analysis of Mycobacterium tuberculosis isolates from HIV-seropositive and HIV-seronegative patients in Kampala, Uganda.BMC infectious diseases · 2009Article
- Evaluation of the new advanced 15-loci MIRU-VNTR genotyping tool in Mycobacterium tuberculosis molecular epidemiology studies.BMC microbiology · 2008Article
- Population-based molecular epidemiological study of tuberculosis in Malatya, Turkey.Journal of clinical microbiology · 2007Article
- Molecular epidemiology of tuberculosis: toy or tool? A review of the literature and examples from Central Europe.Wiener klinische Wochenschrift · 2007Review
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5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While high-copy-number IS6110-based restriction fragment length polymorphism (HCN-RFLP) is the gold standard for typing most Mycobacterium tuberculosis strains, the time taken for culturing and low throughput make it impractical for large-scale prospective typing of large numbers of isolates. The development of a new method, mycobacterial interspersed repetitive units (MIRU), a variation of the original variable-number tandem repeat (VNTR) technique, may provide a viable alternative. Panels based on the original 12-loci MIRU (12MIRU), a combination of 12MIRU and remaining ETR loci (15MIRU-VNTR), and an extended panel with an additional 10 novel regions (25VNTR) were used to study three populations with varying degrees of epidemiological data. MIRU discrimination increased with panel size and the addition of spoligotyping. Combining these two techniques enabled a reduction in the panel size from 25 to 14 loci without a significant loss in discrimination. However, 25VNTR alone or in combination with spoligotyping still possessed weaker discrimination than RFLP for high-copy-number isolates.
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