ArticleJournal of clinical microbiology2006
Comparison of multilocus sequence typing, pulsed-field gel electrophoresis, and antimicrobial susceptibility typing for characterization of Salmonella enterica serotype Newport isolates.
Article in Journal of clinical microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50 citing papers in PubMed.
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- Assessment and Comparison of Molecular Subtyping and Characterization Methods forFrontiers in microbiology · 2019Review
- Comparative analysis of core genome MLST and SNP typing within a European Salmonella serovar Enteritidis outbreak.International journal of food microbiology · 2018Article
- Isolation, Identification, Prevalence, and Genetic Diversity ofFrontiers in microbiology · 2018Article
- Microbial Ecology: Where are we now?Postdoc journal : a journal of postdoctoral research and postdoctoral affairs · 2016Article
- Highly Drug-Resistant Salmonella enterica Serovar Indiana Clinical Isolates Recovered from Broilers and Poultry Workers with Diarrhea in China.Antimicrobial agents and chemotherapy · 2016Article
- Genomic evidence reveals numerous Salmonella enterica serovar Newport reintroduction events in Suwannee watershed irrigation ponds.Applied and environmental microbiology · 2015Article
- Identification and characterization of multidrug-resistant Salmonella enterica serotype Albert isolates in the United States.Antimicrobial agents and chemotherapy · 2015Article
- Community-onset pyomyositis caused by a Salmonella enterica serovar enteritidis sequence type 11 strain producing CTX-M-15 extended-spectrum β-lactamase.Journal of clinical microbiology · 2015Article
- Diversity and antimicrobial resistance of Salmonella enterica isolates from surface water in Southeastern United States.Applied and environmental microbiology · 2014Article
- Multiple-locus variable number of tandem repeats analysis of Salmonella enterica serotype paratyphi A from Yuxi and comparison with isolates from the Chinese Medical Culture Collection Center.Molecular medicine reports · 2014Article
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Abstract
In the United States, multidrug-resistant phenotypes of Salmonella enterica serotype Newport (commonly referred to as MDR-AmpC) have emerged in animals and humans and have become a major public health problem. Although pulsed-field gel electrophoresis (PFGE) is the current "gold standard" typing method for Salmonella, multilocus sequence typing (MLST) may be more relevant to investigations exploring evolutionary and population biology relationships. In this study, 81 Salmonella enterica serotype Newport isolates from humans, food animals, and retail foods were examined for antimicrobial susceptibility and characterized using PFGE and MLST of seven genes, aroC, dnaN, hemD, hisD, purE, sucA, and thrA. Forty-nine percent of the isolates were resistant to nine or more of the tested antimicrobials. Salmonella isolates displayed resistance most often to sulfamethoxazole (57%), streptomycin (56%), tetracycline (56%), ampicillin (52%), and ceftiofur (49%) and, to a lesser extent, to kanamycin (19%), trimethoprim-sulfamethoxazole (17%), and gentamicin (11%). A total of 43 PFGE patterns were generated using XbaI, indicating a genetically diverse population. The largest PFGE cluster contained isolates from clinically ill swine, cattle, and humans. MLST resulted in 12 sequence types (STs), with one type encompassing 62% of the strains. Ten new sequence types and one novel allele type were identified. Furthermore, MLST typing showed that strains closely related by PFGE clustered in major STs, whereas more distantly related strains were separated into two clusters by PFGE. The results of this study demonstrated that the MLST scheme employed here clustered S. enterica serovar Newport isolates in distinct molecular populations, and strain discrimination was enhanced by combining PFGE, antimicrobial susceptibility, and MLST results.
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