ArticleMolecular microbiology2006
Loss of both Holliday junction processing pathways is synthetically lethal in the presence of gonococcal pilin antigenic variation.
Article in Molecular microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 49 citations in OpenAlex.
- Article
- Genetic Manipulation of Neisseria gonorrhoeae and Commensal Neisseria Species.Current protocols · 2024Article
- Article
- PacBio Amplicon Sequencing Method To Measure Pilin Antigenic Variation Frequencies of Neisseria gonorrhoeae.mSphere · 2019Article
- A Double-Strand Break Does Not Promote Neisseria gonorrhoeae Pilin Antigenic Variation.Journal of bacteriology · 2019Article
- Analysis of Pilin Antigenic Variation in Neisseria meningitidis by Next-Generation Sequencing.Journal of bacteriology · 2018Article
- Analyzing Neisseria gonorrhoeae Pilin Antigenic Variation Using 454 Sequencing Technology.Journal of bacteriology · 2016Article
- Antigenic Variation in Bacterial Pathogens.Microbiology spectrum · 2016Article
- Article
- Neisseria gonorrhoeae MutS affects pilin antigenic variation through mismatch correction and not by pilE guanine quartet binding.Journal of bacteriology · 2015Article
- Mobile DNA in the pathogenicMicrobiology spectrum · 2015Article
- Interaction of branch migration translocases with the Holliday junction-resolving enzyme and their implications in Holliday junction resolution.The Journal of biological chemistry · 2014Article
- Characterization of the operon encoding the Holliday junction helicase RuvAB from Mycoplasma genitalium and its role in mgpB and mgpC gene variation.Journal of bacteriology · 2014Article
- Recombination and annealing pathways compete for substrates in making rrn duplications in Salmonella enterica.Genetics · 2014Article
- Constitutively Opa-expressing and Opa-deficient neisseria gonorrhoeae strains differentially stimulate and survive exposure to human neutrophils.Journal of bacteriology · 2013Article
- Neisseria gonorrhoeae RecQ helicase HRDC domains are essential for efficient binding and unwinding of the pilE guanine quartet structure required for pilin antigenic variation.Journal of bacteriology · 2013Article
- The nucleotide excision repair system of Borrelia burgdorferi is the sole pathway involved in repair of DNA damage by UV light.Journal of bacteriology · 2013Article
- Suggested role for G4 DNA in recombinational switching at the antigenic variation locus of the Lyme disease spirochete.PloS one · 2013Article
- Transcription of a cis-acting, noncoding, small RNA is required for pilin antigenic variation in Neisseria gonorrhoeae.PLoS pathogens · 2013Article
- Type IV pilin proteins: versatile molecular modules.Microbiology and molecular biology reviews : MMBR · 2012Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The obligate human pathogen Neisseria gonorrhoeae (Gc) has co-opted conserved recombination pathways to achieve immune evasion by way of antigenic variation (Av). We show that both the RuvABC and RecG Holliday junction (HJ) processing pathways are required for recombinational repair, each can act during genetic transfer, and both are required for pilin Av. Analysis of double mutants shows that either the RecG or RuvAB HJ processing pathway must be functional for normal growth of Gc when RecA is expressed. HJ processing-deficient survivors of RecA expression are enriched for non-piliated bacteria that carry large deletions of the pilE gene. Mutations that prevent pilin variation such as recO, recQ, and a cis-acting pilE transposon insertion all rescue the RecA-dependent growth inhibition of a HJ processing-deficient strain. These results show that pilin Av produces a recombination intermediate that must be processed by either one of the HJ pathways to retain viability, but requires both HJ processing pathways to yield pilin variants. The need for diversity generation through frequent recombination reactions creates a situation where the HJ processing machinery is essential for growth and presents a possible target for novel antimicrobials against gonorrhoea.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.