ArticleNature biotechnology2006
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Article in Nature biotechnology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 323 papers.
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Who cites it
323 citing papers in PubMed, 570 citations in OpenAlex.
- Ectopic overproduction of cell wall glucan through membrane perturbation by an antifungal peptide theonellamide A in fission yeast.iScience · 2026Article
- Gdt1, Gdt2 and the P-type ATPase Pmr1 regulate divalent cations (Ca²⁺ and Mn²⁺) in the fission yeast Schizosaccharomyces pombe.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- The power of many: when genetics met yeasts and high-throughput.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Effects ofExperimental and therapeutic medicine · 2026Article
- The Hemoprotein Hhy1 Promotes Heme-Dependent Catalase Activity of Ctt1.Molecular microbiology · 2026Article
- Nucleosome positioning shapes cryptic antisense transcription.PLoS genetics · 2026Article
- Development of a global screening system for detecting protein-protein interactions by luminescence complementation in fission yeast.Scientific reports · 2026Article
- Rapalink-1 reveals TOR-dependent genes and an agmatinergic axis-based metabolic feedback regulating TOR activity and lifespan in fission yeast.Communications biology · 2025Article
- MoDorado: enhanced detection of tRNA modifications in nanopore sequencing by off-label use of modification callers.Nucleic acids research · 2025Article
- Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions.Scientific reports · 2025Article
- Formation of giant ER sheets by pentadecanoic acid causes lipotoxicity in fission yeast.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- An enzymatic-independent function of palmitoyl hydrolase in cohesin loading onto chromosome.Nucleic acids research · 2025Article
- Fission yeast Caprin protein is required for efficient heterochromatin establishment.PLoS genetics · 2025Article
- Golgi_traff phylogeny reveals ancient eukaryotic genes with recent surprises: replication and diversification of HID1 domain-containing protein unique to Schizosaccharomyces.FEMS microbiology letters · 2025Article
- Review
- [SNG2], a prion form of Cut4/Apc1, confers non-Mendelian inheritance of heterochromatin silencing defect in fission yeast.Nucleic acids research · 2024Article
- Metabolite phosphatase from anhydrobiotic tardigrades.The FEBS journal · 2024Article
- Fission yeast Bsd1 is required for ER stress response in Ire1 independent manner.Molecular biology reports · 2024Article
- Finding new roles of classic biomolecular condensates in the nucleus: Lessons from fission yeast.Cell insight · 2024Review
- DEAD-box ATPase Dbp2 is the key enzyme in an mRNP assembly checkpoint at the 3'-end of genes and involved in the recycling of cleavage factors.Nature communications · 2024Article
263 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cloning of the entire set of an organism's protein-coding open reading frames (ORFs), or 'ORFeome', is a means of connecting the genome to downstream 'omics' applications. Here we report a proteome-scale study of the fission yeast Schizosaccharomyces pombe based on cloning of the ORFeome. Taking advantage of a recombination-based cloning system, we obtained 4,910 ORFs in a form that is readily usable in various analyses. First, we evaluated ORF prediction in the fission yeast genome project by expressing each ORF tagged at the 3' terminus. Next, we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein. Furthermore, using leptomycin B, an inhibitor of the nuclear export protein Crm1, we identified 285 proteins whose localization is regulated by Crm1.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.