ArticleMolecular biology of the cell2006
Increased myosin light chain kinase expression in hypertension: Regulation by serum response factor via an insertion mutation in the promoter.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Application of Single-Cell RNA Sequencing in Ovarian Development.Biomolecules · 2022Review
- Regulation of myosin light-chain phosphorylation and its roles in cardiovascular physiology and pathophysiology.Hypertension research : official journal of the Japanese Society of Hypertension · 2022Review
- Hypoxic modulation of fetal vascular MLCK abundance, localization, and function.American journal of physiology. Regulatory, integrative and comparative physiology · 2021Article
- The Actin-Family Protein Arp4 Is a Novel Suppressor for the Formation and Functions of Nuclear F-Actin.Cells · 2020Article
- Effects of intrauterine growth restriction on CaJournal of physiology and biochemistry · 2020Article
- Visualization of stimulus-specific heterogeneous activation of individual vascular smooth muscle cells in aortic tissues.Journal of cellular physiology · 2018Article
- Myosin light chain kinase controls voltage-dependent calcium channels in vascular smooth muscle.Pflugers Archiv : European journal of physiology · 2014Article
- Article
- Regulation of calcium channels in smooth muscle: new insights into the role of myosin light chain kinase.Channels (Austin, Tex.) · 2014Review
- Regulation of 130-kDa smooth muscle myosin light chain kinase expression by an intronic CArG element.The Journal of biological chemistry · 2013Article
- An intronic MYLK variant associated with inflammatory lung disease regulates promoter activity of the smooth muscle myosin light chain kinase isoform.Journal of molecular medicine (Berlin, Germany) · 2012Article
- Identifying functional single nucleotide polymorphisms in the human CArGome.Physiological genomics · 2011Article
- Role of myosin light chain kinase in regulation of basal blood pressure and maintenance of salt-induced hypertension.American journal of physiology. Heart and circulatory physiology · 2011Article
- A transcribed pseudogene of MYLK promotes cell proliferation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
- Maximal stimulation-induced in situ myosin light chain kinase activity is upregulated in fetal compared with adult ovine carotid arteries.American journal of physiology. Heart and circulatory physiology · 2008Article
- Naturally extended CT . AG repeats increase H-DNA structures and promoter activity in the smooth muscle myosin light chain kinase gene.Molecular and cellular biology · 2008Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Regulation of gene transcription in vascular smooth muscle cells (VSMCs) by serum response factor (SRF) plays a crucial role in vascular development and in the pathophysiology of vascular diseases. Nevertheless, the regulation of specific genes by SRF in vascular diseases is poorly understood. Therefore, we investigated the regulation of smooth muscle myosin light chain kinase (smMLCK) by using spontaneously hypertensive rats (SHR) as an experimental model. We found that smMLCK expression in blood vessels increases during the development of hypertension and is always greater in blood vessels from SHR compared with normotensive rats. Analysis of the DNA sequences of the promoters isolated from SHR and normotensive rats revealed that SHR contain a 12-base pair insertion adjacent to the CArG box. This insertion increases SRF binding to the CArG box and positively regulates SRF-dependent promoter activity. The increase in smMLCK expression was blocked by dominant-negative SRF, dominant-negative Ras, or antisense oligonucleotides to ERK. In vivo, inhibiting MEK decreased smMLCK expression and blood pressure in SHR partly by decreasing SRF binding to the smMLCK promoter. These data provide novel insight into the regulation of smMLCK expression at the molecular level and demonstrate the importance of SRF in regulating smMLCK promoter activity in SHR.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.