ArticleBMC genomics2006
Gene identification and analysis of transcripts differentially regulated in fracture healing by EST sequencing in the domestic sheep.
Article in BMC genomics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Comparative analysis of alternative splicing events in skeletal muscle of different sheep.Heliyon · 2023Article
- Gene markers of fracture healing in early stage and the regulatory mechanism during the process using microarray analysis.Acta orthopaedica et traumatologica turcica · 2016Article
- A polymerase chain reaction-based method for isolating clones from a complimentary DNA library in sheep.Tissue engineering. Part C, Methods · 2014Article
- Effect of temporally patterned TNF-α delivery on in vitro osteogenic differentiation of mesenchymal stem cells cultured on biodegradable polymer scaffolds.Journal of biomaterials science. Polymer edition · 2013Article
- A rapid transcriptome response is associated with desiccation resistance in aerially-exposed killifish embryos.PloS one · 2013Article
- Composite transcriptome assembly of RNA-seq data in a sheep model for delayed bone healing.BMC genomics · 2011Article
- Developmental control of the Nlrp6 inflammasome and a substrate, IL-18, in mammalian intestine.American journal of physiology. Gastrointestinal and liver physiology · 2011Article
- Dose effect of tumor necrosis factor-alpha on in vitro osteogenic differentiation of mesenchymal stem cells on biodegradable polymeric microfiber scaffolds.Biomaterials · 2010Article
- Mutations in GDF5 reveal a key residue mediating BMP inhibition by NOGGIN.PLoS genetics · 2009Article
- Cytogenetic anchoring of radiation hybrid and virtual maps of sheep chromosome X and comparison of X chromosomes in sheep, cattle, and human.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Article
- Modulation of the inflammatory response for enhanced bone tissue regeneration.Tissue engineering. Part B, Reviews · 2008Review
- Surviving extreme polar winters by desiccation: clues from Arctic springtail (Onychiurus arcticus) EST libraries.BMC genomics · 2007Article
- Generation and analysis of expressed sequence tags from the ciliate protozoan parasite Ichthyophthirius multifiliis.BMC genomics · 2007Article
- JUICE: a data management system that facilitates the analysis of large volumes of information in an EST project workflow.BMC bioinformatics · 2006Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe sheep is an important model animal for testing novel fracture treatments and other medical applications. Despite these medical uses and the well known economic and cultural importance of the sheep, relatively little research has been performed into sheep genetics, and DNA sequences are available for only a small number of sheep genes.
resultsIn this work we have sequenced over 47 thousand expressed sequence tags (ESTs) from libraries developed from healing bone in a sheep model of fracture healing. These ESTs were clustered with the previously available 10 thousand sheep ESTs to a total of 19087 contigs with an average length of 603 nucleotides. We used the newly identified sequences to develop RT-PCR assays for 78 sheep genes and measured differential expression during the course of fracture healing between days 7 and 42 postfracture. All genes showed significant shifts at one or more time points. 23 of the genes were differentially expressed between postfracture days 7 and 10, which could reflect an important role for these genes for the initiation of osteogenesis.
conclusionThe sequences we have identified in this work are a valuable resource for future studies on musculoskeletal healing and regeneration using sheep and represent an important head-start for genomic sequencing projects for Ovis aries, with partial or complete sequences being made available for over 5,800 previously unsequenced sheep genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.