ArticleFolia microbiologica2006
Primer R108 performs best in the RAPD strain typing of three Aspergillus species frequently isolated from patients.
Article in Folia microbiologica, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Diversity, molecular phylogeny and fingerprint profiles of airborne Aspergillus species using random amplified polymorphic DNA.World journal of microbiology & biotechnology · 2016Article
- Differentiation of Aspergillus niger by random amplification of polymorphic DNA.Journal of industrial microbiology & biotechnology · 2008Article
- Multilocus sequence typing of the pathogenic fungus Aspergillus fumigatus.Journal of clinical microbiology · 2007Article
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7 authors.
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Abstract
We evaluated the suitability of various primers for the RAPD (random amplified polymorphic DNA) accurate species identification and strain typing of Aspergillus clinical isolates. Five primers described previously were tested for their discriminatory power in three Aspergillus species (A. fumigatus, A. niger agg. and A. flavus - 23 clinical isolates and 2 reference strains). Clustering of RAPD fingerprints corresponded well with the identification based on morphological features. All isolates were resolved as different strains using the primer R108 and the RAPD protocol optimized for a Robocycler thermal cycler. RAPD with the primer R108 thus can be considered to be a valuable, simple and powerful tool for identification and strain delineation of Aspergillus spp.
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