ArticleMolecular systems biology2006
Exploring the regulation of human neural precursor cell differentiation using arrays of signaling microenvironments.
Article in Molecular systems biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
76 citing papers in PubMed, 224 citations in OpenAlex.
- Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications.Bioengineering & translational medicine · 2024Review
- Application and prospects of high-throughput screening forWorld journal of stem cells · 2022Review
- High-Throughput Microenvironment Microarray (MEMA) High-Resolution Imaging.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Human neural organoids: Models for developmental neurobiology and disease.Developmental biology · 2021Review
- Biomaterials by design: Harnessing data for future development.Materials today. Bio · 2021Review
- Stem Cell Niche Microenvironment: Review.Bioengineering (Basel, Switzerland) · 2021Review
- A Single-Layer PDMS Chamber for On-Chip Bacteria Culture.Micromachines · 2020Article
- Emerging trends in modeling human liver diseaseAPL bioengineering · 2019Article
- High-throughput combinatorial screening reveals interactions between signaling molecules that regulate adult neural stem cell fate.Biotechnology and bioengineering · 2019Article
- Advances in Engineered Human Liver Platforms for Drug Metabolism Studies.Drug metabolism and disposition: the biological fate of chemicals · 2018Article
- Designing stem cell niches for differentiation and self-renewal.Journal of the Royal Society, Interface · 2018Review
- Bioengineered Liver Models for Drug Testing and Cell Differentiation Studies.Cellular and molecular gastroenterology and hepatology · 2018Review
- Dynamic inking of large-scale stamps for multiplexed microcontact printing and fabrication of cell microarrays.PloS one · 2018Article
- Pluripotent Stem Cell-Derived Human Tissue: Platforms to Evaluate Drug Metabolism and Safety.The AAPS journal · 2017Review
- A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces.Journal of visualized experiments : JoVE · 2017Article
- High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform.Stem cell reports · 2016Article
- Extracellular matrix microarrays to study inductive signaling for endoderm specification.Acta biomaterialia · 2016Article
- Polymer microarray technology for stem cell engineering.Acta biomaterialia · 2016Review
- Organoids as an in vitro model of human development and disease.Nature cell biology · 2016Review
- Neurogenesis in the Hippocampus of Patients with Temporal Lobe Epilepsy.Current neurology and neuroscience reports · 2016Review
16 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Cells of a developing embryo integrate a complex array of local and long-range signals that act in concert with cell-intrinsic determinants to influence developmental decisions. To systematically investigate the effects of molecular microenvironments on cell fate decisions, we developed an experimental method based on parallel exposure of cells to diverse combinations of extracellular signals followed by quantitative, multi-parameter analysis of cellular responses. Primary human neural precursor cells were captured and cultured on printed microenvironment arrays composed of mixtures of extracellular matrix components, morphogens, and other signaling proteins. Quantitative single cell analysis revealed striking effects of some of these signals on the extent and direction of differentiation. We found that Wnt and Notch co-stimulation could maintain the cells in an undifferentiated-like, proliferative state, whereas bone morphogenetic protein 4 induced an 'indeterminate' differentiation phenotype characterized by simultaneous expression of glial and neuronal markers. Multi-parameter analysis of responses to conflicting signals revealed interactions more complex than previously envisaged including dominance relations that may reflect a cell-intrinsic system for robust specification of responses in complex microenvironments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.