ArticleApplied and environmental microbiology2006
Use of laccase as a novel, versatile reporter system in filamentous fungi.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 49 citations in OpenAlex.
- Development of an efficient protein expression system in the thermophilic fungus Myceliophthora thermophila.Microbial cell factories · 2023Article
- Enhancing the production of a heterologous Trametes laccase (LacA) by replacement of the major cellulase CBH1 in Trichoderma reesei.Journal of industrial microbiology & biotechnology · 2023Article
- A Laccase Gene Reporting System That Enables Genetic Manipulations in a Brown Rot Wood Decomposer FungusMicrobiology spectrum · 2023Article
- Review
- Article
- The small-secreted cysteine-rich protein CyrA is a virulence factor participating in the attack of Caenorhabditis elegans by Duddingtonia flagrans.PLoS pathogens · 2021Article
- Intercellular communication is required for trap formation in the nematode-trapping fungus Duddingtonia flagrans.PLoS genetics · 2019Article
- A Novel Rapid Fungal Promoter Analysis System Using the Phosphopantetheinyl Transferase Gene,Mycobiology · 2018Article
- Immobilization of LccC Laccase from Aspergillus nidulans on Hard Surfaces via Fungal Hydrophobins.Applied and environmental microbiology · 2016Article
- Recombinant expression of a GH12 β-glucanase carrying its own signal peptide from Stachybotrys atra in yeast and filamentous fungi.World journal of microbiology & biotechnology · 2016Article
- Fungal laccases and their applications in bioremediation.Enzyme research · 2014Review
- Biocatalytic potential of laccase-like multicopper oxidases from Aspergillus niger.Microbial cell factories · 2012Article
- Molecular characterization of pathogenic members of the genus Fonsecaea using multilocus analysis.PloS one · 2012Article
- The Aspergillus niger multicopper oxidase family: analysis and overexpression of laccase-like encoding genes.Microbial cell factories · 2011Article
- Evolution of multicopper oxidase genes in coprophilous and non-coprophilous members of the order sordariales.Current genomics · 2011Article
- LccA, an archaeal laccase secreted as a highly stable glycoprotein into the extracellular medium by Haloferax volcanii.Applied and environmental microbiology · 2010Article
- Heterologous laccase production and its role in industrial applications.Bioengineered bugsReview
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Authors and funding
19 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laccases are copper-containing enzymes which oxidize phenolic substrates and transfer the electrons to oxygen. Many filamentous fungi contain several laccase-encoding genes, but their biological roles are mostly not well understood. The main interest in laccases in biotechnology is their potential to be used to detoxify phenolic substances. We report here on a novel application of laccases as a reporter system in fungi. We purified a laccase enzyme from the ligno-cellulolytic ascomycete Stachybotrys chartarum. It oxidized the artificial substrate 2,2'-azino-di-(3-ethylbenzthiazolinsulfonate) (ABTS). The corresponding gene was isolated and expressed in Aspergillus nidulans, Aspergillus niger, and Trichoderma reesei. Heterologously expressed laccase activity was monitored in colorimetric enzyme assays and on agar plates with ABTS as a substrate. The use of laccase as a reporter was shown in a genetic screen for the isolation of improved T. reesei cellulase production strains. In addition to the laccase from S. charatarum, we tested the application of three laccases from A. nidulans (LccB, LccC, and LccD) as reporters. Whereas LccC oxidized ABTS (Km = 0.3 mM), LccD did not react with ABTS but with DMA/ADBP (3,5-dimethylaniline/4-amino-2,6-dibromophenol). LccB reacted with DMA/ADBP and showed weak activity with ABTS. The different catalytic properties of LccC and LccD allow simultaneous use of these two laccases as reporters in one fungal strain.
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