ArticleApplied and environmental microbiology2006
Functional heterogeneity of RpoS in stress tolerance of enterohemorrhagic Escherichia coli strains.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 68 citations in OpenAlex.
- The Viable but Non-Culturable (VBNC) State in Vibrio Species: Why Studying the VBNC State Now Is More Exciting than Ever.Advances in experimental medicine and biology · 2023Article
- Expression of stress regulator and virulence genes ofFood science and biotechnology · 2020Article
- Viable but Nonculturable Escherichia coli O157:H7 and Salmonella enterica in Fresh Produce: Rapid Determination by Loop-Mediated Isothermal Amplification Coupled with a Propidium Monoazide Treatment.Applied and environmental microbiology · 2020Article
- Function, Evolution, and Composition of the RpoS Regulon inFrontiers in microbiology · 2020Review
- Article
- Survival ofApplied and environmental microbiology · 2019Article
- Evaluation of the inflammatory response to Kudoa septempunctata genotype ST3 isolated from olive flounder (Paralichthys olivaceus) in Caco-2 cells.Parasite (Paris, France) · 2018Article
- Article
- Genome-Wide Transcriptional Response to Varying RpoS Levels in Escherichia coli K-12.Journal of bacteriology · 2017Article
- Current Perspectives on Viable but Non-culturable State in Foodborne Pathogens.Frontiers in microbiology · 2017Review
- Phenotypic and Genotypic Features of aFrontiers in veterinary science · 2017Article
- Diversity of Survival Patterns among Escherichia coli O157:H7 Genotypes Subjected to Food-Related Stress Conditions.Frontiers in microbiology · 2016Article
- Growth media simulating ileal and colonic environments affect the intracellular proteome and carbon fluxes of enterohemorrhagic Escherichia coli O157:H7 strain EDL933.Applied and environmental microbiology · 2013Article
- Escherichia coli lacking RpoS are rare in natural populations of non-pathogens.G3 (Bethesda, Md.) · 2012Article
- Culture history and population heterogeneity as determinants of bacterial adaptation: the adaptomics of a single environmental transition.Microbiology and molecular biology reviews : MMBR · 2012Review
- Polymorphisms in rpoS and stress tolerance heterogeneity in natural isolates of Cronobacter sakazakii.Applied and environmental microbiology · 2012Article
- New insights into bacterial adaptation through in vivo and in silico experimental evolution.Nature reviews. Microbiology · 2012Review
- Distinct transcriptional profiles and phenotypes exhibited by Escherichia coli O157:H7 isolates related to the 2006 spinach-associated outbreak.Applied and environmental microbiology · 2012Article
- Phenotypic diversity caused by differential RpoS activity among environmental Escherichia coli isolates.Applied and environmental microbiology · 2011Article
- A systems biology approach sheds new light on Escherichia coli acid resistance.Nucleic acids research · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The stationary-phase sigma factor (RpoS) regulates many cellular responses to environmental stress conditions such as heat, acid, and alkali shocks. On the other hand, mutations at the rpoS locus have frequently been detected among pathogenic as well as commensal strains of Escherichia coli. The objective of this study was to perform a functional analysis of the RpoS-mediated stress responses of enterohemorrhagic E. coli strains from food-borne outbreaks. E. coli strains belonging to serotypes O157:H7, O111:H11, and O26:H11 exhibited polymorphisms for two phenotypes widely used to monitor rpoS mutations, heat tolerance and glycogen synthesis, as well as for two others, alkali tolerance and adherence to Caco-2 cells. However, these strains synthesized the oxidative acid resistance system through an rpoS-dependent pathway. During the transition from mildly acidic growth conditions (pH 5.5) to alkaline stress (pH 10.2), cell survival was dependent on rpoS functionality. Some strains were able to overcome negative regulation by RpoS and induced higher beta-galactosidase activity without compromising their acid resistance. There were no major differences in the DNA sequences in the rpoS coding regions among the tested strains. The heterogeneity of rpoS-dependent phenotypes observed for stress-related phenotypes was also evident in the Caco-2 cell adherence assay. Wild-type O157:H7 strains with native rpoS were less adherent than rpoS-complemented counterpart strains, suggesting that rpoS functionality is needed. These results show that some pathogenic E. coli strains can maintain their acid tolerance capability while compromising other RpoS-dependent stress responses. Such adaptation processes may have significant impact on a pathogen's survival in food processing environments, as well in the host's stomach and intestine.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.