ArticleApplied and environmental microbiology2006
Facile recovery of individual high-molecular-weight, low-copy-number natural plasmids for genomic sequencing.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- Novel insights into the genome organization ofMicrobial genomics · 2025Article
- Three Distinct Annotation Platforms Differ in Detection of Antimicrobial Resistance Genes in Long-Read, Short-Read, and Hybrid Sequences Derived from Total Genomic DNA or from Purified Plasmid DNA.Antibiotics (Basel, Switzerland) · 2022Article
- Article
- Characterization of KPC-Encoding Plasmids from Enterobacteriaceae Isolated in a Czech Hospital.Antimicrobial agents and chemotherapy · 2018Article
- Characterization of the Complete Nucleotide Sequences of IncA/CAntimicrobial agents and chemotherapy · 2017Article
- Genetic Background and Expression of the NewFrontiers in microbiology · 2017Article
- Article
- Complete genome sequence, lifestyle, and multi-drug resistance of the human pathogen Corynebacterium resistens DSM 45100 isolated from blood samples of a leukemia patient.BMC genomics · 2012Article
- Identification of Enterococcus faecalis bacteria resistant to heavy metals and antibiotics in surface waters of the Mololoa River in Tepic, Nayarit, Mexico.Environmental monitoring and assessment · 2011Article
- Major families of multiresistant plasmids from geographically and epidemiologically diverse staphylococci.G3 (Bethesda, Md.) · 2011Article
- Genome signatures of Escherichia coli O157:H7 isolates from the bovine host reservoir.Applied and environmental microbiology · 2011Article
- rmtD2, a new allele of a 16S rRNA methylase gene, has been present in Enterobacteriaceae isolates from Argentina for more than a decade.Antimicrobial agents and chemotherapy · 2011Article
- The two-component signal transduction system CopRS of Corynebacterium glutamicum is required for adaptation to copper-excess stress.PloS one · 2011Article
- Sequence of pNL194, a 79.3-kilobase IncN plasmid carrying the blaVIM-1 metallo-beta-lactamase gene in Klebsiella pneumoniae.Antimicrobial agents and chemotherapy · 2010Article
- Emergence of resistance among USA300 methicillin-resistant Staphylococcus aureus isolates causing invasive disease in the United States.Antimicrobial agents and chemotherapy · 2010Article
- ISEcp1-mediated transposition and homologous recombination can explain the context of bla(CTX-M-62) linked to qnrB2.Antimicrobial agents and chemotherapy · 2010Article
- The Citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic Escherichia coli.Journal of bacteriology · 2010Article
- Comparative genomics of the IncA/C multidrug resistance plasmid family.Journal of bacteriology · 2009Article
- The RepA_N replicons of Gram-positive bacteria: a family of broadly distributed but narrow host range plasmids.Plasmid · 2009Review
- Multidrug resistance in bacteria.Annual review of biochemistry · 2009Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequencing of the large (>50 kb), low-copy-number (<5 per cell) plasmids that mediate horizontal gene transfer has been hindered by the difficulty and expense of isolating DNA from individual plasmids of this class. We report here that a kit method previously devised for purification of bacterial artificial chromosomes (BACs) can be adapted for effective preparation of individual plasmids up to 220 kb from wild gram-negative and gram-positive bacteria. Individual plasmid DNA recovered from less than 10 ml of Escherichia coli, Staphylococcus, and Corynebacterium cultures was of sufficient quantity and quality for construction of high-coverage libraries, as shown by sequencing five native plasmids ranging in size from 30 kb to 94 kb. We also report recommendations for vector screening to optimize plasmid sequence assembly, preliminary annotation of novel plasmid genomes, and insights on mobile genetic element biology derived from these sequences. Adaptation of this BAC method for large plasmid isolation removes one major technical hurdle to expanding our knowledge of the natural plasmid gene pool.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.