ArticleApplied and environmental microbiology2006
Old yellow enzymes protect against acrolein toxicity in the yeast Saccharomyces cerevisiae.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 90 citations in OpenAlex.
- Disorders of Redox Homeostasis and Its Importance in Acrolein Toxicity.International journal of molecular sciences · 2025Article
- Machine learning reveals genes impacting oxidative stress resistance across yeasts.Nature communications · 2025Article
- Genome-wide analysis ofFrontiers in plant science · 2025Article
- Hydrogen sulfide production during early yeast fermentation correlates with volatile sulfur compound biogenesis but not thiol release.FEMS yeast research · 2023Article
- Article
- Unbalance between Pyridine Nucleotide Cofactors in The SOD1 Deficient YeastInternational journal of molecular sciences · 2022Article
- Disruption of multiple copies of the Prostaglandin F2alpha synthase gene affects oxidative stress response and infectivity in Trypanosoma cruzi.PLoS neglected tropical diseases · 2022Article
- Gene Coexpression Connectivity Predicts Gene Targets Underlying High Ionic-Liquid Tolerance in Yarrowia lipolytica.mSystems · 2022Article
- Discovery and characterization of a terpene biosynthetic pathway featuring a norbornene-forming Diels-Alderase.Nature communications · 2022Article
- Ethanol stress responses in Kluyveromyces marxianus: current knowledge and perspectives.Applied microbiology and biotechnology · 2022Review
- Genetic and molecular characterization of multicomponent resistance ofLife science alliance · 2020Article
- Engineered mitochondrial production of monoterpenes in Saccharomyces cerevisiae.Metabolic engineering · 2019Article
- Natural allelic variations of Saccharomyces cerevisiae impact stuck fermentation due to the combined effect of ethanol and temperature; a QTL-mapping study.BMC genomics · 2019Article
- Acrolein-stressed threshold adaptation alters the molecular and metabolic bases of an engineered Saccharomyces cerevisiae to improve glutathione production.Scientific reports · 2018Article
- Old Yellow Enzyme fromFrontiers in immunology · 2018Article
- Engineering the biocatalytic selectivity of iridoid production in Saccharomyces cerevisiae.Metabolic engineering · 2017Article
- Disrupting the cortical actin cytoskeleton points to two distinct mechanisms of yeast [PSI+] prion formation.PLoS genetics · 2017Article
- Dynamic control of ERG20 expression combined with minimized endogenous downstream metabolism contributes to the improvement of geraniol production in Saccharomyces cerevisiae.Microbial cell factories · 2017Article
- Acrolein-Induced Oxidative Stress and Cell Death Exhibiting Features of Apoptosis in the Yeast Saccharomyces cerevisiae Deficient in SOD1.Cell biochemistry and biophysics · 2015Article
- Comprehensive genome-wide analysis reveals different classes of enigmatic old yellow enzyme in fungi.Scientific reports · 2014Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Acrolein is a ubiquitous reactive aldehyde which is formed as a product of lipid peroxidation in biological systems. In this present study, we screened the complete set of viable deletion strains in Saccharomyces cerevisiae for sensitivity to acrolein to identify cell functions involved in resistance to reactive aldehydes. We identified 128 mutants whose gene products are localized throughout the cell. Acrolein-sensitive mutants were distributed among most major biological processes but particularly affected gene expression, metabolism, and cellular signaling. Surprisingly, the screen did not identify any antioxidants or similar stress-protective molecules, indicating that acrolein toxicity may not be mediated via reactive oxygen species. Most strikingly, a mutant lacking an old yellow enzyme (OYE2) was identified as being acrolein sensitive. Old yellow enzymes are known to reduce alpha,beta-unsaturated carbonyl compounds in vitro, but their physiological roles have remained uncertain. We show that mutants lacking OYE2, but not OYE3, are sensitive to acrolein, and overexpression of both isoenzymes increases acrolein tolerance. Our data indicate that OYE2 is required for basal levels of tolerance, whereas OYE3 expression is particularly induced following acrolein stress. Despite the range of alpha,beta-unsaturated carbonyl compounds that have been identified as substrates of old yellow enzymes in vitro, we show that old yellow enzymes specifically mediate resistance to small alpha,beta-unsaturated carbonyl compounds, such as acrolein, in vivo.
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Registered trials
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