ArticleApplied and environmental microbiology2006
A protective immune response is generated in rainbow trout by an OmpH-like surface antigen (P18) of Flavobacterium psychrophilum.
Article in Applied and environmental microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 48 citations in OpenAlex.
- Current Challenges of Vaccination in Fish Health Management.Animals : an open access journal from MDPI · 2024Review
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- Genome-Wide Subtraction Analysis and Reverse Vaccinology to Detect Novel Drug Targets and Potential Vaccine Candidates Against Ehrlichia chaffeensis.Applied biochemistry and biotechnology · 2023Article
- In the Search of Potential Serodiagnostic Proteins to Discriminate Between Acute and Chronic Q Fever in Humans. Some Promising Outcomes.Frontiers in cellular and infection microbiology · 2020Article
- Chemical Synthesis and Functional Analysis of VarvA Cyclotide.Molecules (Basel, Switzerland) · 2018Article
- Involvement of an Skp-Like Protein, PGN_0300, in the Type IX Secretion System of Porphyromonas gingivalis.Infection and immunity · 2016Article
- Flavobacterium psychrophilum vaccine development: a difficult task.Microbial biotechnology · 2014Review
- Characterization of the miiuy croaker (Miichthys miiuy) transcriptome and development of immune-relevant genes and molecular markers.PloS one · 2014Article
- Molecular cloning, characterization and expression analysis of a CC chemokine gene from miiuy croaker (Miichthys miiuy).Fish physiology and biochemistry · 2012Article
- Potential serodiagnostic markers for Q fever identified in Coxiella burnetii by immunoproteomic and protein microarray approaches.BMC microbiology · 2012Article
- Identification of cold-temperature-regulated genes in Flavobacterium psychrophilum.Applied and environmental microbiology · 2011Article
- Identification and analysis of immune-related transcriptome in Asian seabass Lates calcarifer.BMC genomics · 2010Article
- Francisella tularensis T-cell antigen identification using humanized HLA-DR4 transgenic mice.Clinical and vaccine immunology : CVI · 2010Article
- Identification of protein candidates for the serodiagnosis of Q fever endocarditis by an immunoproteomic approach.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2009Article
- Population structure of the fish-pathogenic bacterium Flavobacterium psychrophilum.Applied and environmental microbiology · 2008Article
- Challenges associated with heterologous expression of Flavobacterium psychrophilum proteins in Escherichia coli.Marine biotechnology (New York, N.Y.)Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Investigations of the surface characteristics of Flavobacterium psychrophilum, an important pathogen of fish, assisted us in identifying a surface protein termed P18. In the current study, we developed a simple and efficient procedure for the purification of this protein by a two-step method. First, P18 was selectively released from flavobacteria by a heat-HEPES treatment of the cells and then subjected to anion-exchange high-performance liquid chromatography. De novo sequencing was used to generate a fragmented peptide spectrum from purified P18. Comparison of two obtained peptide sequences with a partial genome sequence of F. psychrophilum (INRA, Jouy-en-Josas, France) identified one gene encoding a 166-amino-acid OmpH-like protein that mostly likely undergoes N-terminal cleavage of the 23-residue signal peptide. The susceptibility of the OmpH-like protein to proteinase K treatment and the bacteriostatic/bactericidal activities of anti-OmpH-like protein antibodies indicated that this protein is actually exposed on the surface of F. psychrophilum. Vaccination trials showed that the OmpH-like protein can induce a high titer of anti-OmpH-like protein antibodies which are protective. Taken together, these results suggest that this surface protein produced by F. psychrophilum could be used in future vaccine development as a promising candidate antigen.
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