ArticleBMC genomics2006
Seven different genes encode a diverse mixture of isoforms of Bet v 1, the major birch pollen allergen.
Article in BMC genomics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Insights into structural and binding studies of pollen allergen Bet v 1 using computational approaches.In silico pharmacology · 2025Article
- To stay or not to stay intact as an allergen: the endolysosomal degradation assay used as tool to analyze protein immunogenicity andFrontiers in allergy · 2024Review
- Plant Stress Scenarios Differentially Affect Expression and IgE Reactivity of Grass Group-1 Allergen (β-Expansin) in Maize and Rice Pollen.Frontiers in allergy · 2022Article
- Tiered approach for the identification of Mal d 1 reduced, well tolerated apple genotypes.Scientific reports · 2020Article
- Article
- Conformational Flexibility Differentiates Naturally Occurring Bet v 1 Isoforms.International journal of molecular sciences · 2017Article
- Molecular Cloning and Expression Analysis of hyp-1 Type PR-10 Family Genes in Hypericum perforatum.Frontiers in plant science · 2016Article
- Long-Read Single Molecule Sequencing to Resolve Tandem Gene Copies: The Mst77Y Region on the Drosophila melanogaster Y Chromosome.G3 (Bethesda, Md.) · 2015Article
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- Article
- Novel birch pollen specific immunotherapy formulation based on contiguous overlapping peptides.Clinical and translational allergy · 2013Article
- Crystallographically mapped ligand binding differs in high and low IgE binding isoforms of birch pollen allergen bet v 1.Journal of molecular biology · 2012Article
- The Y-segment of novel cold dehydrin genes is conserved and codons in the PR-10 genes are under positive selection in Oxytropis (Fabaceae) from contrasting climates.Molecular genetics and genomics : MGG · 2012Article
- Article
- Characterisation of the Vitis vinifera PR10 multigene family.BMC plant biology · 2010Article
- Antigen aggregation decides the fate of the allergic immune response.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen.BMC plant biology · 2009Article
- Genomic characterization of putative allergen genes in peach/almond and their synteny with apple.BMC genomics · 2008Article
- Assessment of allelic diversity in intron-containing Mal d 1 genes and their association to apple allergenicity.BMC plant biology · 2008Article
- Comparative genomics of fungal allergens and epitopes shows widespread distribution of closely related allergen and epitope orthologues.BMC genomics · 2006Article
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4 authors.
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Abstract
backgroundPollen of the European white birch (Betula pendula, syn. B. verrucosa) is an important cause of hay fever. The main allergen is Bet v 1, member of the pathogenesis-related class 10 (PR-10) multigene family. To establish the number of PR-10/Bet v 1 genes and the isoform diversity within a single tree, PCR amplification, cloning and sequencing of PR-10 genes was performed on two diploid B. pendula cultivars and one interspecific tetraploid Betula hybrid. Sequences were attributed to putative genes based on sequence identity and intron length. Information on transcription was derived by comparison with homologous cDNA sequences available in GenBank/EMBL/DDJB. PCR-cloning of multigene families is accompanied by a high risk for the occurrence of PCR recombination artifacts. We screened for and excluded these artifacts, and also detected putative artifact sequences among database sequences.
resultsForty-four different PR-10 sequences were recovered from B. pendula and assigned to thirteen putative genes. Sequence homology suggests that three genes were transcribed in somatic tissue and seven genes in pollen. The transcription of three other genes remains unknown. In total, fourteen different Bet v 1-type isoforms were identified in the three cultivars, of which nine isoforms were entirely new. Isoforms with high and low IgE-reactivity are encoded by different genes and one birch pollen grain has the genetic background to produce a mixture of isoforms with varying IgE-reactivity. Allergen diversity is even higher in the interspecific tetraploid hybrid, consistent with the presence of two genomes.
conclusionIsoforms of the major birch allergen Bet v 1 are encoded by multiple genes, and we propose to name them accordingly. The present characterization of the Bet v 1 genes provides a framework for the screening of specific Bet v 1 genes among other B. pendula cultivars or Betula species, and for future breeding for trees with a reduced allergenicity. Investigations towards sensitization and immunotherapy should anticipate that patients are exposed to a mixture of Bet v 1 isoforms of different IgE-reactivity, even if pollen originates from a single birch tree.
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