ArticlePediatrics2006
Real-time polymerase chain reaction for the rapid detection of group B streptococcal colonization in neonates.
Article in Pediatrics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 45 citations in OpenAlex.
- Hypervirulent Streptococcus agalactiae septicemia in twin ex-premature infants transmitted by breast milk: report of source detection and isolate characterization using commonly available molecular diagnostic methods.Annals of clinical microbiology and antimicrobials · 2020Article
- Highly Sensitive and Rapid Identification ofFrontiers in microbiology · 2020Article
- Microbiological comparison of blood culture and amplification of 16S rDNA methods in combination with DGGE for detection of neonatal sepsis in blood samples.European journal of pediatrics · 2018Article
- Mucosal vaccination promotes clearance of Streptococcus agalactiae vaginal colonization.Vaccine · 2017Article
- Gastric fluid versus amniotic fluid analysis for the identification of intra-amniotic infection due to Ureaplasma species.The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians · 2016Article
- Direct molecular versus culture-based assessment of Gram-positive cocci in biopsies of patients with major abscesses and diabetic foot infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Xpert GBS assay for rapid detection of group B streptococcus in gastric fluid samples from newborns.Journal of clinical microbiology · 2014Article
- Real-time PCR assay provides reliable assessment of intrapartum carriage of group B Streptococcus.Journal of clinical microbiology · 2010Article
- Group B streptococcus and early-onset sepsis in the era of maternal prophylaxis.Pediatric clinics of North America · 2009Review
- Induction and termination of inflammatory signaling in group B streptococcal sepsis.Immunological reviews · 2008Review
- Diagnosis of neonatal group B Streptococcus sepsis by nested-PCR of residual urine samples.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2008Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundGroup B streptococcal (GBS) infection remains a leading cause of neonatal sepsis. Currently, the management guidelines of neonates born to women with unknown GBS status at delivery are unclear. In this cohort, who undergo at least a 48-hour observation, a rapid method of detection of GBS colonization would allow targeted evaluation and treatment, as well as prevent delayed discharge.
objectiveThe goal of this research was to evaluate the validity of rapid fluorescent real-time polymerase chain reaction in comparison with standard culture to detect GBS colonization in infants born to women whose GBS status is unknown at delivery. DESIGN/
methodsNeonates at >32 weeks' gestation born to women whose GBS status was unknown at delivery were included. Samples were obtained from the ear, nose, rectum, and gastric aspirate for immediate culture and real-time polymerase chain reaction after DNA extraction using the LightCycler. Melting point curves were generated, and confirmatory agar gel electrophoresis was performed.
resultsThe study population (n = 94) had a mean +/- SD gestational age of 38 +/- 2 weeks and birth weight of 3002 +/- 548 g. The rates of GBS colonization by culture were 17% and 51% by real-time polymerase chain reaction. The 4 surface sites had comparable rates of GBS. The overall sensitivities, specificities, and positive and negative predictive values of real-time polymerase chain reaction were: 90%, 80.3%, 28%, and 98.9%.
conclusionsReal-time polymerase chain reaction resulted in a threefold higher rate of detection of GBS colonization and had an excellent negative predictive value in a cohort of neonates with unknown maternal GBS status at delivery. Thus, real-time polymerase chain reaction would be a useful clinical tool in the management of those infants potentially at risk for invasive GBS infection and would allow earlier discharge for those found to be not at risk.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.