ArticleJournal of bacteriology2006
An unstable competence-induced protein, CoiA, promotes processing of donor DNA after uptake during genetic transformation in Streptococcus pneumoniae.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Two D-loop resolution systems enable natural genetic transformation in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Article
- Competence shut-off by intracellular pheromone degradation in salivarius streptococci.PLoS genetics · 2022Article
- The alternative sigma factor σeLife · 2020Article
- A Review of the Functional Annotations of Important Genes in the AHPND-Causing pVA1 Plasmid.Microorganisms · 2020Review
- Refining the Pneumococcal Competence Regulon by RNA Sequencing.Journal of bacteriology · 2019Article
- Draft Genome Sequences of 12 Dry-Heat-Resistant Bacillus Strains Isolated from the Cleanrooms Where the Viking Spacecraft Were Assembled.Genome announcements · 2018Article
- Competence for Genetic Transformation in Streptococcus pneumoniae: Mutations in σA Bypass the ComW Requirement for Late Gene Expression.Journal of bacteriology · 2016Article
- Temporal Regulation of the Transformasome and Competence Development inFrontiers in microbiology · 2016Article
- Direct involvement of DprA, the transformation-dedicated RecA loader, in the shut-off of pneumococcal competence.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Exit from competence for genetic transformation in Streptococcus pneumoniae is regulated at multiple levels.PloS one · 2013Article
- Sequence, structure and functional diversity of PD-(D/E)XK phosphodiesterase superfamily.Nucleic acids research · 2012Article
- Comparative analyses of Campylobacter concisus strains reveal the genome of the reference strain BAA-1457 is not representative of the species.Gut pathogens · 2011Article
- Compensatory evolution of pbp mutations restores the fitness cost imposed by β-lactam resistance in Streptococcus pneumoniae.PLoS pathogens · 2011Article
- Competence for genetic transformation in Streptococcus pneumoniae: termination of activity of the alternative sigma factor ComX is independent of proteolysis of ComX and ComW.Journal of bacteriology · 2009Article
- Identification of the major protein component of the pneumococcal eclipse complex.Journal of bacteriology · 2007Article
- Article
- The cell pole: the site of cross talk between the DNA uptake and genetic recombination machinery.Critical reviews in biochemistry and molecular biologyReview
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Natural genetic transformation in Streptococcus pneumoniae entails transcriptional activation of at least two sets of genes. One set of genes, activated by the competence-specific response regulator ComE, is involved in initiating competence, whereas a second set is activated by the competence-specific alternative sigma factor ComX and functions in DNA uptake and recombination. Here we report an initial characterization of CoiA, a ComX-dependent gene product that is induced during competence and is required for transformation. CoiA is widely conserved among gram-positive bacteria, and in streptococci, the entire coiA locus composed of four genes is conserved. By use of immunoblot assay, we show that, similar to its message, CoiA protein is transient, appearing at 10 min and largely disappearing by 30 min post-competence induction. Using complementation analysis, we establish that coiA is the only gene of this induced locus needed for transformability. We find no indication of CoiA having a role in regulating competence. Finally, using 32P- and 3H-labeled donor DNA, we demonstrate that a coiA mutant can internalize normal amounts of donor DNA compared to the wild-type strain but is unable to process it into viable transformants, suggesting a role for CoiA after DNA uptake, either in DNA processing or recombination.
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