ArticleJournal of bacteriology2006
Molecular characterization of Pantoea stewartii subsp. stewartii HrpY, a conserved response regulator of the Hrp type III secretion system, and its interaction with the hrpS promoter.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Uncovering virulence factors inApplied and environmental microbiology · 2023Article
- Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP-PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. savastanoi DAPP-PG 722.Molecular plant pathology · 2021Article
- A Re-evaluation of the Taxonomy and Classification of the Type III Secretion System in a Pathogenic Bacterium Causing Soft Rot Disease of Pleurotus eryngii.Current microbiology · 2021Article
- Integration of multiple stimuli-sensing systems to regulate HrpS and type III secretion system in Erwinia amylovora.Molecular genetics and genomics : MGG · 2018Article
- Analysis of thePeerJ · 2017Article
- Transcriptional Regulation by the Short-Chain Fatty Acyl Coenzyme A Regulator (ScfR) PccR Controls Propionyl Coenzyme A Assimilation by Rhodobacter sphaeroides.Journal of bacteriology · 2015Article
- Identification and characterization of VpsR and VpsT binding sites in Vibrio cholerae.Journal of bacteriology · 2015Article
- Derivative of plant phenolic compound inhibits the type III secretion system of Dickeya dadantii via HrpX/HrpY two-component signal transduction and Rsm systems.Molecular plant pathology · 2015Article
- Proteomic analysis of the quorum-sensing regulon in Pantoea stewartii and identification of direct targets of EsaR.Applied and environmental microbiology · 2013Article
- The bacterium Pantoea stewartii uses two different type III secretion systems to colonize its plant host and insect vector.Applied and environmental microbiology · 2012Article
- Pantoea stewartii subsp. stewartii: lessons learned from a xylem-dwelling pathogen of sweet corn.Molecular plant pathology · 2011Article
- Identification and functional characterization of the iron-dependent regulator (IdeR) of Mycobacterium avium subsp. paratuberculosis.Microbiology (Reading, England) · 2009Article
- Multiple activities of the plant pathogen type III effector proteins WtsE and AvrE require WxxxE motifs.Molecular plant-microbe interactions : MPMI · 2009Article
- Systems level analysis of two-component signal transduction systems in Erwinia amylovora: role in virulence, regulation of amylovoran biosynthesis and swarming motility.BMC genomics · 2009Article
- Genome-wide analysis of the PreA/PreB (QseB/QseC) regulon of Salmonella enterica serovar Typhimurium.BMC microbiology · 2009Article
- The Zur of Xanthomonas campestris functions as a repressor and an activator of putative zinc homeostasis genes via recognizing two distinct sequences within its target promoters.Nucleic acids research · 2008Article
- Identification of the DNA bases of a DNase I footprint by the use of dye primer sequencing on an automated capillary DNA analysis instrument.Journal of biomolecular techniques : JBT · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pantoea stewartii subsp. stewartii is a bacterial pathogen of corn. Its pathogenicity depends on the translocation of effector proteins into host cells by the Hrp type III secretion system. We previously showed by genetic analysis that the HrpX sensor kinase and the HrpY response regulator are at the head of a complex cascade of regulators controlling hrp/hrc secretion and wts effector genes. This cascade also includes the HrpS response regulator and the HrpL alternative sigma factor. These regulators are shared among many important plant pathogens in the genera Pantoea, Erwinia, and Pseudomonas. In this study, we dissect the regulatory elements in the hrpS promoter region, using genetic and biochemical approaches, and show how it integrates various environmental signals, only some of which are dependent on phosphorylation of HrpY. Primer extension located the transcriptional start site of hrpS at a sigma70 promoter 601 bp upstream of the open reading frame. Electrophoretic mobility shift assays and DNase I footprinting analysis demonstrated that HrpY binds to conserved regulatory elements immediately adjacent to this promoter, and its binding affinity was increased by phosphorylation at D57. A consensus sequence for the two direct repeats bound by HrpY is proposed. Deletion analysis of the promoter region revealed that both the HrpY binding site and additional sequences farther upstream, including a putative integration host factor binding site, are required for hrpS expression. This finding suggests that other unknown regulatory proteins may act cooperatively with HrpY.
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