ArticleJournal of bacteriology2006
Genetic evidence for the requirement of RecA loading activity in SOS induction after UV irradiation in Escherichia coli.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Chromosome Segregation and Cell Division Defects inMicroorganisms · 2023Article
- RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in liveJournal of the Royal Society, Interface · 2022Article
- Regulation of Cell Division in Bacteria by Monitoring Genome Integrity and DNA Replication Status.Journal of bacteriology · 2020Review
- A bacterial DNA repair pathway specific to a natural antibiotic.Molecular microbiology · 2019Article
- DdcA antagonizes a bacterial DNA damage checkpoint.Molecular microbiology · 2019Article
- CRISPR-Cas adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonized by 5' ssDNA exonucleases.Nucleic acids research · 2018Article
- Functional fine-tuning between bacterial DNA recombination initiation and quality control systems.PloS one · 2018Article
- Article
- Double-Strand Break Repair and Holliday Junction Processing Are Required for Chromosome Processing in Stationary-Phase Escherichia coli Cells.G3 (Bethesda, Md.) · 2011Article
- Genetic requirements for high constitutive SOS expression in recA730 mutants of Escherichia coli.Journal of bacteriology · 2011Article
- Roles of PriA protein and double-strand DNA break repair functions in UV-induced restriction alleviation in Escherichia coli.Genetics · 2006Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The SOS response in Escherichia coli results in the coordinately induced expression of more than 40 genes which occurs when cells are treated with DNA-damaging agents. This response is dependent on RecA (coprotease), LexA (repressor), and the presence of single-stranded DNA (ssDNA). A prerequisite for SOS induction is the formation of a RecA-ssDNA filament. Depending on the DNA substrate, the RecA-ssDNA filament is produced by either RecBCD, RecFOR, or a hybrid recombination mechanism with specific enzyme activities, including helicase, exonuclease, and RecA loading. In this study we examined the role of RecA loading activity in SOS induction after UV irradiation. We performed a genetic analysis of SOS induction in strains with a mutation which eliminates RecA loading activity in the RecBCD enzyme (recB1080 allele). We found that RecA loading activity is essential for SOS induction. In the recB1080 mutant RecQ helicase is not important, whereas RecJ nuclease slightly decreases SOS induction after UV irradiation. In addition, we found that the recB1080 mutant exhibited constitutive expression of the SOS regulon. Surprisingly, this constitutive SOS expression was dependent on the RecJ protein but not on RecFOR, implying that there is a different mechanism of RecA loading for constitutive SOS expression.
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