ArticlePlant physiology2006
Rapid, futile K+ cycling and pool-size dynamics define low-affinity potassium transport in barley.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 71 citations in OpenAlex.
- Article
- Determination of optimal NHFrontiers in plant science · 2023Article
- Differential Effects of Ammonium (NHPlants (Basel, Switzerland) · 2022Article
- Review
- The transcriptome variations of Panaxnotoginseng roots treated with different forms of nitrogen fertilizers.BMC genomics · 2019Article
- Comparison between Arabidopsis and Rice for Main Pathways of K(+) and Na(+) Uptake by Roots.Frontiers in plant science · 2016Review
- Effects of high NH(+) 4 on K(+) uptake, culm mechanical strength and grain filling in wheat.Frontiers in plant science · 2014Article
- A pharmacological analysis of high-affinity sodium transport in barley (Hordeum vulgare L.): a 24Na+/42K+ study.Journal of experimental botany · 2012Article
- Isolation and characterization of a novel ammonium overly sensitive mutant, amos2, in Arabidopsis thaliana.Planta · 2012Article
- Effects of nitrogen form on growth, CO₂ assimilation, chlorophyll fluorescence, and photosynthetic electron allocation in cucumber and rice plants.Journal of Zhejiang University. Science. B · 2011Article
- Competition between uptake of ammonium and potassium in barley and Arabidopsis roots: molecular mechanisms and physiological consequences.Journal of experimental botany · 2010Article
- Tracing cationic nutrients from xylem into stem tissue of French bean by stable isotope tracers and cryo-secondary ion mass spectrometry.Plant physiology · 2010Article
- Non-reciprocal interactions between K+ and Na+ ions in barley (Hordeum vulgare L.).Journal of experimental botany · 2008Article
- NH4+-stimulated and -inhibited components of K+ transport in rice (Oryza sativa L.).Journal of experimental botany · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using the short-lived radiotracer 42K+, we present a comprehensive subcellular flux analysis of low-affinity K+ transport in plants. We overturn the paradigm of cytosolic K+ pool-size homeostasis and demonstrate that low-affinity K+ transport is characterized by futile cycling of K+ at the plasma membrane. Using two methods of compartmental analysis in intact seedlings of barley (Hordeum vulgare L. cv Klondike), we present data for steady-state unidirectional influx, efflux, net flux, cytosolic pool size, and exchange kinetics, and show that, with increasing external [K+] ([K+]ext), both influx and efflux increase dramatically, and that the ratio of efflux to influx exceeds 70% at [K+]ext > or = 20 mm. Increasing [K+]ext, furthermore, leads to a shortening of the half-time for cytosolic K+ exchange, to values 2 to 3 times lower than are characteristic of high-affinity transport. Cytosolic K+ concentrations are shown to vary between 40 and 200 mm, depending on [K+]ext, on nitrogen treatment (NO3- or NH4+), and on the dominant mode of transport (high- or low-affinity transport), illustrating the dynamic nature of the cytosolic K+ pool, rather than its homeostatic maintenance. Based on measurements of trans-plasma membrane electrical potential, estimates of cytosolic K+ pool size, and the magnitude of unidirectional K+ fluxes, we describe efflux as the most energetically demanding of the cellular K+ fluxes that constitute low-affinity transport.
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