Evidence map›Paper›PMID 16810766›Full record

ArticleWorld journal of gastroenterology2006

Whole-cell recordings of calcium and potassium currents in acutely isolated smooth muscle cells.

Qing Cai, Zhong-Liang Zhu, Xiao-Li Fan

Abstract read
In one paragraph

Article in World journal of gastroenterology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Qing CaiDepartment of Physiology and Pathophysiology, Medical School of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
Zhong-Liang Zhu
Xiao-Li Fan

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo record calcium and potassium currents in acutely isolated smooth muscle cells of mesenteric arterial branches in rats.

methodsSmooth muscle cells were freshly isolated by collagenase digest and mechanical trituration with polished pipettes. Patch clamp technique in whole-cell mode was employed to record calcium and potassium currents.

resultsThe procedure dissociated smooth muscle cells without impairing the electrophysiological characteristics of the cells. The voltage-gated Ca2+ and potassium currents were successfully recorded using whole-cell patch clamp configuration.

conclusionThe method dissociates smooth muscle cells from rat mesenteric arterial branches. Voltage-gated channel currents can be recorded in this preparation.

Indexed as

AnimalsCalciumCalcium ChannelsCell SeparationDelayed Rectifier Potassium ChannelsMaleMesenteric ArteriesMyocytes, Smooth MusclePatch-Clamp TechniquesPotassiumRatsRats, Sprague-DawleyCalciumCalcium ChannelsDelayed Rectifier Potassium ChannelsPotassium

Identifiers

PMID16810766
PMCPMC4087728

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.