ArticleWorld journal of gastroenterology2006
Differentiation of human umbilical cord blood stem cells into hepatocytes in vivo and in vitro.
Article in World journal of gastroenterology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 42 citations in OpenAlex.
- The making of cells and organs in xenogeneic animal bioreactor.Signal transduction and targeted therapy · 2026Review
- Review
- DNA barcode to trace the development and differentiation of cord blood stem cells (Review).Molecular medicine reports · 2021Review
- Application of Cord Blood and Cord Blood-Derived Induced Pluripotent Stem Cells for Cartilage Regeneration.Cell transplantation · 2019Review
- Comparative analysis of human UCB and adipose tissue derived mesenchymal stem cells for their differentiation potential into brown and white adipocytes.Molecular biology reports · 2018Article
- Effect of human umbilical cord blood stem cell transplantation on oval cell response in 2-AAF/CCL4 liver injury model: experimental immunohistochemical study.Inflammation and regeneration · 2017Article
- Stemness-related transcriptional factors and homing gene expression profiles in hepatic differentiation and cancer.Molecular medicine (Cambridge, Mass.) · 2016Article
- The hematopoietic system in the context of regenerative medicine.Methods (San Diego, Calif.) · 2016Review
- In Vitro and In Vivo Hepatic Differentiation of Adult Somatic Stem Cells and Extraembryonic Stem Cells for Treating End Stage Liver Diseases.Stem cells international · 2015Review
- Beneficial vasoactive endothelial effects of fluvastatin: focus on prostacyclin and nitric oxide.Heart and vessels · 2011Article
- Bone marrow stem cells and liver regeneration.Experimental hematology · 2010Review
- Migration of human umbilical cord blood cells into rat liver.International journal of stem cells · 2010Article
- Future of cord blood for non-oncology uses.Bone marrow transplantation · 2009Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo study the condition and potentiality of human umbilical cord blood stem cells (HUCBSC) to differentiate into hepatocytes in vivo or in vitro.
methodsIn a cell culture study of human umbilical cord blood stem cell (HUCBSC) differentiation, human umbilical cord blood mononuclear cells (HUCBMNC) were separated by density gradient centrifugation. Fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) and the supernatant of fetal liver were added in the inducing groups. Only FGF was added in the control group. The expansion and differentiation of HUCBMNC in each group were observed. Human alpha fetoprotein (AFP) and albumin (ALB) were detected by immunohistochemistry. In the animal experiments, the survival SD rats with acute hepatic injury after carbon tetrachloride (CCL4) injection 48 h were randomly divided into three groups. The rats in group A were treated with human umbilical cord blood serum. The rats in group B were treated with HUCBMNC transplantation. The rats in group C were treated with HUCBMNC transplantation followed by intraperitoneal cyclophosphamide for 7 d. The rats were killed at different time points after the treatment and the liver tissue was histopathologically studied and human AFP and ALB detected by immunohistochemistry. The human X inactive-specific transcript gene fragment in the liver tissue was amplified by PCR to find human DNA.
resultsThe results of cell culture showed that adherent cells were stained negative for AFP or ALB in control group. However, the adherent cells in the inducing groups stained positive for AFP or ALB. The result of animal experiment showed that no human AFP or ALB positive cells present in the liver tissue of group A (control group). However, many human AFP or ALB positive cells were scattered around sinus hepaticus and the central veins of hepatic lobules and in the portal area in group B and group C after one month. The fragment of human X chromagene could be detected in the liver tissue of groups B and C, but not in group A.
conclusionUnder certain conditions HUCBSC can differentiate into liver cells in vivo and in vitro.
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