ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2006
Dissection of the nuclear genome of barley by chromosome flow sorting.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 54 citations in OpenAlex.
- Introgression of barley chromosome arms 4H and 6H into wheat via Robertsonian translocations: GBS-assisted structural analysis and impact on grain nutrient composition.Plant molecular biology · 2026Article
- A flow cytometry-based analysis to establish a cell cycle synchronization protocol for Saccharum spp.Scientific reports · 2020Article
- Molecular cytogenetic and morphological characterization of two wheat-barley translocation lines.PloS one · 2018Article
- Addition of Manas barley chromosome arms to the hexaploid wheat genome.BMC genetics · 2016Article
- Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113.PloS one · 2016Article
- Sequencing of 15 622 gene-bearing BACs clarifies the gene-dense regions of the barley genome.The Plant journal : for cell and molecular biology · 2015Article
- The subtelomeric region is important for chromosome recognition and pairing during meiosis.Scientific reports · 2014Article
- Flow sorting and sequencing meadow fescue chromosome 4F.Plant physiology · 2013Article
- PCR and sequence analysis of barley chromosome 2H subjected to the gametocidal action of chromosome 2C.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Conserved synteny-based anchoring of the barley genome physical map.Functional & integrative genomics · 2013Article
- Chromosomes in the flow to simplify genome analysis.Functional & integrative genomics · 2012Review
- Integration of genetic and physical maps of the chickpea (Cicer arietinum L.) genome using flow-sorted chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- Frequent gene movement and pseudogene evolution is common to the large and complex genomes of wheat, barley, and their relatives.The Plant cell · 2011Article
- Unlocking the barley genome by chromosomal and comparative genomics.The Plant cell · 2011Article
- Specific patterns of gene space organisation revealed in wheat by using the combination of barley and wheat genomic resources.BMC genomics · 2010Article
- Gene content and virtual gene order of barley chromosome 1H.Plant physiology · 2009Article
- Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley.BMC genomics · 2008Article
- A novel resource for genomics of Triticeae: BAC library specific for the short arm of rye (Secale cereale L.) chromosome 1R (1RS).BMC genomics · 2008Article
- Chromosome-based genomics in the cereals.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2007Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isolation of mitotic chromosomes using flow cytometry is an attractive way to dissect nuclear genomes into their individual chromosomal components or portions of them. This approach is especially useful in plants with complex genomes, where it offers a targeted and hence economical approach to genome analysis and gene cloning. In several plant species, DNA of flow-sorted chromosomes has been used for isolation of molecular markers from specific genome regions, for physical mapping using polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH), for integration of genetic and physical maps and for construction of chromosome-specific DNA libraries, including those cloned in bacterial artificial chromosome vectors. Until now, chromosome analysis and sorting using flow cytometry (flow cytogenetics) has found little application in barley (2n = 14, 1C approximately 5,100 Mbp) because of the impossibility of discriminating and sorting individual chromosomes, except for the smallest chromosome 1H and some translocation chromosomes with DNA content significantly different from the remaining chromosomes. In this work, we demonstrate that wheat-barley ditelosomic addition lines can be used to sort any arm of barley chromosomes 2H-7H. Thus, the barley genome can be dissected into fractions representing only about 6-12% of the total genome. This advance makes the flow cytogenetics an attractive tool, which may greatly facilitate genome analysis and gene cloning in barley.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.