ArticleJournal of cancer research and clinical oncology2006
Epigenetic silencing of the putative tumor suppressor gene testisin in testicular germ cell tumors.
Article in Journal of cancer research and clinical oncology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Maternal plasma folate impacts differential DNA methylation in an epigenome-wide meta-analysis of newborns.Nature communications · 2016Pooled it
- A multi-analyte liquid biopsy approach for nonseminomatous testicular germ cell tumors: combining cfDNA and N-glycan analysis in blood and seminal plasma.Cancer cell international · 2025Article
- In Search of TGCT Biomarkers: A ComprehensiveDisease markers · 2020Article
- Cell surface-anchored serine proteases in cancer progression and metastasis.Cancer metastasis reviews · 2019Review
- Genome wide DNA methylation profiles provide clues to the origin and pathogenesis of germ cell tumors.PloS one · 2015Article
- Article
- Promoter methylation and tissue-specific transcription of the α7 nicotinic receptor gene, CHRNA7.Journal of molecular neuroscience : MN · 2012Article
- Membrane-anchored serine proteases in health and disease.Progress in molecular biology and translational science · 2011Review
- The cutting edge: membrane-anchored serine protease activities in the pericellular microenvironment.The Biochemical journal · 2010Review
- DNA methylation of cancer genome.Birth defects research. Part C, Embryo today : reviews · 2009Review
- Discovery of DNA methylation markers in cervical cancer using relaxation ranking.BMC medical genomics · 2008Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTestisin, a serine protease abundantly expressed only in normal testes, is thought to be a tumor suppressor gene silenced by aberrant methylation in testicular germ cell tumors (TGCT). This study aimed at identifying the CpG sites relevant for testisin gene silencing when methylated and evaluating the potential of aberrant methylation as a biomarker in TGCT.
methodsBisulfite sequencing and subsequent real-time RT-PCR were carried out in germ cell tumor cell lines and a cervical carcinoma cell line to reveal CpG sites implicated in testisin gene silencing. The normalized index of methylation (NIM) and the relative gene expression (RGE) were calculated in 33 TGCT and 19 normal testicular tissue samples by quantitative methylation-specific PCR (QMSP) and real-time RT-PCR.
resultsCpG sites in the 5'untranslated region proved to be relevant for testisin gene silencing when methylated. Targeting these CpG sites by QMSP, we demonstrated that the median NIM was 8.6 times higher in the TGCT group than in normal testicular samples. This was associated with a median 23.6 times lower RGE in the TGCT probes. Non-seminomas had a significantly higher NIM than seminomas, but RGE was downregulated to comparable levels in both subgroups. QMSP was highly sensitive and distinguished TGCT from normal samples with excellent specificity.
conclusionsOur results demonstrate for the first time that aberrant methylation of specific CpG sites near the transcription initiation site is an important factor in testisin gene silencing in TGCT.
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