ArticleThe EMBO journal2006
Calcium-dependent release of NO from intracellular S-nitrosothiols.
Article in The EMBO journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 52 citations in OpenAlex.
- Neuronal nitric oxide synthase expression in secretory cells of the human parotid and submandibular salivary glands: evidence from light and electron microscopy.Cell and tissue research · 2026Article
- Driver Mutations of Pancreatic Cancer Affect CaFunction (Oxford, England) · 2023Article
- Biological Mechanisms ofAntioxidants (Basel, Switzerland) · 2021Review
- NADPH diaphorase detects S-nitrosylated proteins in aldehyde-treated biological tissues.Scientific reports · 2020Article
- RhoA S-nitrosylation as a regulatory mechanism influencing endothelial barrier function in response to GBiochemical pharmacology · 2017Article
- Nitric oxide signals are interlinked with calcium signals in normal pancreatic stellate cells upon oxidative stress and inflammation.Open biology · 2016Article
- Nitric oxide is required for the auxin-induced activation of NADPH-dependent thioredoxin reductase and protein denitrosylation during root growth responses in arabidopsis.Annals of botany · 2015Article
- Novel lipophilic probe for detecting near-membrane reactive oxygen species responses and its application for studies of pancreatic acinar cells: effects of pyocyanin and L-ornithine.Antioxidants & redox signaling · 2015Article
- Expression of human cationic trypsinogen (PRSS1) in murine acinar cells promotes pancreatitis and apoptotic cell death.Cell death & disease · 2014Article
- Kinetic analysis of DAF-FM activation by NO: toward calibration of a NO-sensitive fluorescent dye.Nitric oxide : biology and chemistry · 2013Article
- Region-specific changes in activities of cell death-related proteases and nitric oxide metabolism in rat brain in a chronic unpredictable stress model.Metabolic brain disease · 2012Article
- Mitochondrial function and malfunction in the pathophysiology of pancreatitis.Pflugers Archiv : European journal of physiology · 2012Review
- N-methyl-D-aspartate receptor-dependent denitrosylation of neuronal nitric oxide synthase increase the enzyme activity.PloS one · 2012Article
- Selective killing of cancer cells by a small molecule targeting the stress response to ROS.Nature · 2011Article
- TRPV3 regulates nitric oxide synthase-independent nitric oxide synthesis in the skin.Nature communications · 2011Article
- Hippocampal neuronal nitric oxide synthase mediates the stress-related depressive behaviors of glucocorticoids by downregulating glucocorticoid receptor.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Activation of GluR6-containing kainate receptors induces ubiquitin-dependent Bcl-2 degradation via denitrosylation in the rat hippocampus after kainate treatment.The Journal of biological chemistry · 2011Article
- S-nitrosothiol signals in the enteric nervous system: lessons learnt from big brother.Frontiers in neuroscience · 2011Article
- Release and elementary mechanisms of nitric oxide in hair cells.Journal of neurophysiology · 2010Article
- S-nitrosylation in cardiovascular signaling.Circulation research · 2010Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The paper describes a novel cellular mechanism for rapid calcium-dependent nitric oxide (NO) release. This release occurs due to NO liberation from S-nitrosothiols. We have analysed the changes of NO concentration in acutely isolated pancreatic acinar cells. Supramaximal acetylcholine (ACh) stimulation induced a Ca(2+)-dependent increase in the fluorescence in the majority of cells loaded with the NO probe DAF-FM via a patch pipette. The ACh-induced NO signals were insensitive to inhibitors of calmodulin and protein kinase C but were inhibited by calpain antagonists. The initial part of the NO signals induced by 10 muM ACh showed little sensitivity to inhibition of NO synthase (NOS); however, cell pretreatment with NO donors (increasing cellular S-nitrosothiol contents) substantially enhanced the initial component of NO responses. Pancreatic acinar cells were able to generate fast calcium-dependent NO responses when stimulated with physiological or supramaximal doses of secretagogues. Importantly, the source of this NO is the already available S-nitrosothiol store rather than de novo synthesis by NOS. A similar mechanism of NO release was found in dorsal root ganglia neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.