ArticleRNA (New York, N.Y.)2006
A three-hybrid screen identifies mRNAs controlled by a regulatory protein.
Article in RNA (New York, N.Y.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Decoding RNA-protein interactions using high-throughput methods.RNA biology · 2026Review
- PUF3 RNA binding protein ofHeliyon · 2024Article
- RNA regulatory networks diversified through curvature of the PUF protein scaffold.Nature communications · 2015Article
- Ehd4 encodes a novel and Oryza-genus-specific regulator of photoperiodic flowering in rice.PLoS genetics · 2013Article
- A eukaryotic translation initiation factor 4E-binding protein promotes mRNA decapping and is required for PUF repression.Molecular and cellular biology · 2012Article
- Binding of the 5'-untranslated region of coronavirus RNA to zinc finger CCHC-type and RNA-binding motif 1 enhances viral replication and transcription.Nucleic acids research · 2012Article
- Review
- Using the Yeast Three-Hybrid System to Identify Proteins that Interact with a Phloem-Mobile mRNA.Frontiers in plant science · 2012Article
- In silico characterization and prediction of global protein-mRNA interactions in yeast.Nucleic acids research · 2011Article
- Nutrients and the Pkh1/2 and Pkc1 protein kinases control mRNA decay and P-body assembly in yeast.The Journal of biological chemistry · 2011Article
- Translational repression by PUF proteins in vitro.RNA (New York, N.Y.) · 2010Article
- Selections that optimize RNA display in the yeast three-hybrid system.RNA (New York, N.Y.) · 2010Review
- A 5' cytosine binding pocket in Puf3p specifies regulation of mitochondrial mRNAs.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Article
- A single C. elegans PUF protein binds RNA in multiple modes.RNA (New York, N.Y.) · 2009Article
- RNA-binding proteins and post-transcriptional gene regulation.FEBS letters · 2008Review
- Basis of altered RNA-binding specificity by PUF proteins revealed by crystal structures of yeast Puf4p.Nature structural & molecular biology · 2008Article
- Mpt5p, a stress tolerance- and lifespan-promoting PUF protein in Saccharomyces cerevisiae, acts upstream of the cell wall integrity pathway.Eukaryotic cell · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA-protein interactions are important in many biological contexts. Identification of the networks that connect regulatory proteins to one another and to the mRNAs they control is a critical need. Here, we use a yeast three-hybrid screening approach to identify RNAs that bind a known RNA regulatory protein, the Saccharomyces cerevisiae PUF protein, Mpt5p. The assay selects RNAs that bind in vivo using simple phenotypes and reporter genes. It enables rapid analyses of the affinity and specificity of the interaction. We show that the method identifies mRNAs that are genuinely regulated by the protein in vivo, and that it complements biochemical strategies, yielding a set of mRNAs that overlap with, but are distinct from, those obtained by biochemical means. The approach we describe facilitates construction of protein-RNA linkage maps.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.