ArticleThe Journal of physiology2006
Glutamate acts at NMDA receptors on fresh bovine and on cultured human retinal pigment epithelial cells to trigger release of ATP.
Article in The Journal of physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- Review of the clinical electrooculogram - Part 1: Mechanism of the Light-Rise.Documenta ophthalmologica. Advances in ophthalmology · 2026Review
- Implication ofInternational journal of molecular sciences · 2021Article
- Article
- Purinergic signaling in the retina: From development to disease.Brain research bulletin · 2019Review
- Thrombin induces CaMolecular vision · 2019Article
- Stimulation of TLR3 triggers release of lysosomal ATP in astrocytes and epithelial cells that requires TRPML1 channels.Scientific reports · 2018Article
- Targeting the tight junction protein, zonula occludens-1, with the connexin43 mimetic peptide, αCT1, reduces VEGF-dependent RPE pathophysiology.Journal of molecular medicine (Berlin, Germany) · 2017Article
- Expression of anoctamins in retinal pigment epithelium (RPE).Pflugers Archiv : European journal of physiology · 2016Article
- Central GPR109A Activation Mediates Glutamate-Dependent Pressor Response in Conscious Rats.The Journal of pharmacology and experimental therapeutics · 2016Article
- Involvement of nucleotides in glial growth following scratch injury in avian retinal cell monolayer cultures.Purinergic signalling · 2015Article
- Article
- The bizarre pharmacology of the ATP release channel pannexin1.Neuropharmacology · 2013Review
- Lysosomal alkalinization, lipid oxidation, and reduced phagosome clearance triggered by activation of the P2X7 receptor.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Communication between corneal epithelial cells and trigeminal neurons is facilitated by purinergic (P2) and glutamatergic receptors.PloS one · 2012Article
- Elevated pressure triggers a physiological release of ATP from the retina: Possible role for pannexin hemichannels.Neuroscience · 2008Article
- Probenecid, a gout remedy, inhibits pannexin 1 channels.American journal of physiology. Cell physiology · 2008Article
- Müller glia as an active compartment modulating nervous activity in the vertebrate retina: neurotransmitters and trophic factors.Neurochemical research · 2008Review
- Purinergic signalling in the subretinal space: a role in the communication between the retina and the RPE.Purinergic signalling · 2008Article
- Restoration of lysosomal pH in RPE cells from cultured human and ABCA4(-/-) mice: pharmacologic approaches and functional recovery.Investigative ophthalmology & visual science · 2008Article
- Potential therapeutic interest of adenosine A2A receptors in psychiatric disorders.Current pharmaceutical design · 2008Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The photoreceptors lie between the inner retina and the retinal pigment epithelium (RPE). The release of glutamate by the phototoreceptors can signal changes in light levels to inner retinal neurons, but the role of glutamate in communicating with the RPE is unknown. Since RPE cells are known to release ATP, we asked whether glutamate could trigger ATP release from RPE cells and whether this altered cell signalling. Stimulation of the apical face of fresh bovine RPE eyecups with 100 mum NMDA increased ATP levels more than threefold, indicating that both receptors for NMDA and release of ATP occurred across the apical membrane of fresh RPE cells. NMDA increased ATP levels bathing cultured human ARPE-19 cells more than twofold, with NMDA receptor inhibitors MK-801 and d-AP5 preventing this release. Blocking the glycine site of the NMDA receptor with 5,7-dichlorokynurenic acid prevented ATP release from ARPE-19 cells. Release was also blocked by channel blocker NPPB and Ca(2+) chelator BAPTA, but not by cystic fibrosis transmembrane conductance regulator (CFTR) blocker glibenclamide or vesicular release inhibitor brefeldin A. Glutamate produced a dose-dependent release of ATP from ARPE-19 cells that was substantially inhibited by MK-801. NMDA triggered a rise in cell Ca(2+) that was blocked by MK-801, by the ATPase apyrase, by the P2Y(1) receptor antagonist MRS2179 and by depletion of intracellular Ca(2+) stores with thapsigargin. These results suggest that glutamate stimulates NMDA receptors on the apical membrane of RPE cells to release ATP. This secondary release can amplify the glutaminergic signal by increasing Ca(2+) inside RPE cells, and might activate Ca(2+)-dependent conductances. The interplay between glutaminergic and purinergic systems may thus be important for light-dependent interactions between photoreceptors and the RPE.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.