ReviewHistochemistry and cell biology2006
Lock and roll: single-molecule genotyping in situ using padlock probes and rolling-circle amplification.
Review in Histochemistry and cell biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 88 citations in OpenAlex.
- A sensitive one-pot ROA assay for rapid miRNA detection.aBIOTECH · 2024Article
- Rolling Circle Amplification in Integrated Microsystems: An Uncut Gem toward Massively Multiplexed Pathogen Diagnostics and Genotyping.Accounts of chemical research · 2021Review
- Diagnosis for COVID-19: current status and future prospects.Expert review of molecular diagnostics · 2021Review
- COVID-19 Pandemic: Review of Contemporary and Forthcoming Detection Tools.Infection and drug resistance · 2021Review
- Detection of Prorocentrum minimum by hyperbranched rolling circle amplification coupled with lateral flow dipstick.Environmental science and pollution research international · 2020Article
- Highly sensitive and specific screening of EGFR mutation using a PNA microarray-based fluorometric assay based on rolling circle amplification and graphene oxide.RSC advances · 2019Article
- Histochemistry and Cell Biology: 61 years and not tired at all.Histochemistry and cell biology · 2019Article
- Article
- Rapid Identification of Seven Waterborne Exophiala Species by RCA DNA Padlock Probes.Mycopathologia · 2018Article
- Rolling circle amplification as isothermal gene amplification in molecular diagnostics.Biochip journal · 2016Review
- Use of Padlock Probes and Rolling Circle Amplification (RCA) for Rapid Identification of Trichophyton Species, Related to Human and Animal Disorder.Jundishapur journal of microbiology · 2015Article
- Use of rolling circle amplification to rapidly identify species of Cladophialophora potentially causing human infection.Mycopathologia · 2013Article
- Fluorescence imaging of single-copy DNA sequences within the human genome using PNA-directed padlock probe assembly.Chemistry & biology · 2013Article
- Single-molecule fluorescence in situ hybridization: quantitative imaging of single RNA molecules.BMB reports · 2013Review
- Molecular identification of Clonorchis sinensis and discrimination with other opisthorchid liver fluke species using multiple Ligation-depended Probe Amplification (MLPA).Parasites & vectors · 2011Article
- Ultrasensitive detection of low-abundance surface-marker protein using isothermal rolling circle amplification in a microfluidic nanoliter platform.Small (Weinheim an der Bergstrasse, Germany) · 2011Article
- PNA-based microbial pathogen identification and resistance marker detection: An accurate, isothermal rapid assay based on genome-specific features.Artificial DNA, PNA & XNA · 2010Article
- PNA-based microbial pathogen identification and resistance marker detection: an accurate, isothermal rapid assay based on genome-specific features.Artificial DNA, PNA & XNA · 2010Article
- Rapid detection of ERG11 gene mutations in clinical Candida albicans isolates with reduced susceptibility to fluconazole by rolling circle amplification and DNA sequencing.BMC microbiology · 2009Article
- Specific detection of prostate cancer cells in urine by multiplex immunofluorescence cytology.Human pathology · 2009Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this review I will describe the development of a technique that enables genotyping of individual DNA molecules in the context of morphologically preserved fixed cells, from the fundamental concept published in 1994 to the present status. The review describes enzyme-assisted histochemistry approaches to achieve highly specific molecular identification reactions coupled to efficient signal amplification. The primary molecular identification is accomplished through circularization of oligonucleotide probes, called padlock probes. The circularization reaction is catalyzed by a DNA ligase, which provides robust distinction between single-nucleotide variants under standard reaction conditions. To generate a detectable signal from individual circularized probe molecules, a DNA polymerase is added that replicates probe circles, generating a long tandem-repeated DNA product, easily visualized using a standard epi-fluorescence microscope. Individual signals are recorded as bright dots, providing digital information about the abundance of specific sequences and opportunities for simultaneous detection of several targets using spectral multiplexing. The importance of strictly target-dependent signal amplification will be discussed.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.