Evidence map›Paper›PMID 16804705›Full record

ArticleMycorrhiza2006

Expression of the autofluorescent protein, DsRed2, in the recombinants of the ectomycorrhizal basidiomycete, Suillus grevillei, generated by Agrobacterium-mediated transformation.

Hitoshi Murata, Masahide Sunagawa, Takashi Yamazaki, Kazuo Shishido, Tomohiro Igasaki

Abstract read
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In one paragraph

Article in Mycorrhiza, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Hitoshi MurataDepartment of Applied Microbiology and Mushroom Science, Forestry & Forest Products Research Institute, Matsunosato 1, Tsukuba, 305-8687, Japan. murmur@ffpri.affrc.go.jp.
Masahide SunagawaDepartment of Applied Microbiology and Mushroom Science, Forestry & Forest Products Research Institute, Matsunosato 1, Tsukuba, 305-8687, Japan.
Takashi YamazakiDepartment of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa, 226-8501, Japan.
Kazuo ShishidoDepartment of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa, 226-8501, Japan.
Tomohiro IgasakiDepartment of Molecular Cell Biology, Forestry & Forest Products Research Institute, Matsunosato 1, Tsukuba, 305-8687, Japan.
Forestry and Forest Products Research Institute · JPTokyo Institute of Technology · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recombinants were generated from the ectomycorrhizal basidiomycete, Suillus grevillei, through agroinfection using a binary vector carrying the hygromycin B resistance and the autofluorescent protein, DsRed2, markers. DsRed2 was driven by a cis-regulatory region of the glyceraldeyde-3-phosphate dehydrogenase gene (gpd) from the wood-rotting basidiomycete, Coriolus hirsutus, which contains promoters and 5' gpd sequences with first through fourth exons and expressed for the first time in Suillus spp. The transformation system and recombinants expressing an autofluorescent protein may be useful in genetic analysis of the symbiosis.

Indexed as

BasidiomycotaDNA, FungalGenetic VectorsLuminescent ProteinsMicroscopy, FluorescenceMycorrhizaePolymerase Chain ReactionRhizobiumTransformation, GeneticDNA, Fungalfluorescent protein 583Luminescent Proteins

Identifiers

PMID16804705
OpenAlexW2084298620

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.