ArticleLab on a chip2006
Measurement of cell migration in response to an evolving radial chemokine gradient triggered by a microvalve.
Article in Lab on a chip, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Vat photopolymerization 3D printed microfluidic devices for organ-on-a-chip applications.Lab on a chip · 2023Review
- On-Chip Micro Mixer Driven by Elastic Wall with Virtual Actuator.Micromachines · 2021Article
- Push/Pull Inequality Based High-Speed On-Chip Mixer Enhanced by Wettability.Micromachines · 2020Article
- Light-inducible activation of cell cycle progression in Xenopus egg extracts under microfluidic confinement.Lab on a chip · 2019Article
- 3D-printed Quake-style microvalves and micropumps.Lab on a chip · 2018Article
- On-Chip Quantitative Measurement of Mechanical Stresses During Cell Migration with Emulsion Droplets.Scientific reports · 2016Article
- Microfluidic gradient device for studying mesothelial cell migration and the effect of chronic carbon nanotube exposure.Journal of micromechanics and microengineering : structures, devices, and systems · 2015Article
- Gradient generation platforms: new directions for an established microfluidic technology.Lab on a chip · 2014Article
- Microfluidic transwell inserts for generation of tissue culture-friendly gradients in well plates.Lab on a chip · 2014Article
- Gradient biomaterials and their influences on cell migration.Interface focus · 2012Article
- Image-based analysis of primary human neutrophil chemotaxis in an automated direct-viewing assay.Journal of immunological methods · 2011Article
- Microfluidic quadrupole and floating concentration gradient.Nature communications · 2011Article
- Design and dynamic characterization of "single-stroke" peristaltic PDMS micropumps.Lab on a chip · 2011Article
- A low resistance microfluidic system for the creation of stable concentration gradients in a defined 3D microenvironment.Biomedical microdevices · 2010Article
- Directional decisions during neutrophil chemotaxis inside bifurcating channels.Integrative biology : quantitative biosciences from nano to macro · 2010Article
- Microfluidic technologies for temporal perturbations of chemotaxis.Annual review of biomedical engineering · 2010Review
- Article
- A microfluidic imaging chamber for the direct observation of chemotactic transmigration.Biomedical microdevices · 2010Article
- Polydimethylsiloxane-based conducting composites and their applications in microfluidic chip fabrication.Biomicrofluidics · 2009Article
- Evaluation of neurite outgrowth anisotropy using a novel application of circular analysis.Journal of neuroscience methods · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
We describe a novel chemotaxis assay based on the microvalve-actuated release of a chemoattractant from a cell-free microchamber into a cell-containing microchamber. The microvalve chemotaxis device (microVCD) was placed on the stage of a conventional inverted microscope to obtain time-lapse micrographs of neutrophils migrating in a radially-symmetric evolving gradient of the chemotactic factor CXCL8/Interleukin-8. A fluorescent tracer was added to the CXCL8 solution to visualize the evolution of the gradient profile, so that at each time point the cell positions could be assigned CXCL8 concentration values. Tracking of individual neutrophils for 90 minutes showed that (a) the neutrophil migratory response is, on average, radially directed towards the CXCL8 source; (b) significant non-radial displacements occur frequently; and (c) there is considerable heterogeneity in the migration speeds and directions amongst the neutrophil population. A custom-made imaging analysis tool was used to extract measurements of migratory behavior such as speed, velocity along the gradient's radial axis, and the cosine of the turning angle as a function of CXCL8 concentration. The microVCD can be easily adapted to study the migratory behavior of cultured cells other than neutrophils.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.