ArticleThe Journal of cell biology2006
Microclusters of inhibitory killer immunoglobulin-like receptor signaling at natural killer cell immunological synapses.
Article in The Journal of cell biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed, 106 citations in OpenAlex.
- Immunological synapse: structures, molecular mechanisms and therapeutic implications in disease.Signal transduction and targeted therapy · 2025Review
- Induced CD8α identifies human NK cells with enhanced proliferative fitness and modulates NK cell activation.The Journal of clinical investigation · 2024Article
- Human natural killer cells: Form, function, and development.The Journal of allergy and clinical immunology · 2023Review
- KIR signaling is regulated by electrostatic interaction of its cytosolic tail with the plasma membrane despite being neutral polyampholyte.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Blockade of the immunosuppressive KIR2DL5/PVR pathway elicits potent human NK cell-mediated antitumor immunity.The Journal of clinical investigation · 2022Article
- Nanoscale Colocalization of NK Cell Activating and Inhibitory Receptors Controls Signal Integration.Frontiers in immunology · 2022Article
- The Role of the Cytoskeleton in Regulating the Natural Killer Cell Immune Response in Health and Disease: From Signaling Dynamics to Function.Frontiers in cell and developmental biology · 2021Review
- Natural Killer Cells: Tumor Surveillance and Signaling.Cancers · 2020Review
- Genetic diversity affects the nanoscale membrane organization and signaling of natural killer cell receptors.Science signaling · 2019Article
- CD8αα homodimers function as a coreceptor for KIR3DL1.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Spatial Clustering of Receptors and Signaling Molecules Regulates NK Cell Response to Peptide Repertoire Changes.Frontiers in immunology · 2019Article
- Review
- T cell immunoengineering with advanced biomaterials.Integrative biology : quantitative biosciences from nano to macro · 2017Review
- Molecular Imaging: A Useful Tool for the Development of Natural Killer Cell-Based Immunotherapies.Frontiers in immunology · 2017Review
- A lifetime in photochemistry; some ultrafast measurements on singlet states.Proceedings. Mathematical, physical, and engineering sciences · 2016Review
- The Size of Activating and Inhibitory Killer Ig-like Receptor Nanoclusters Is Controlled by the Transmembrane Sequence and Affects Signaling.Cell reports · 2016Article
- Suppressing the killer instinct.Science signaling · 2016Article
- Simultaneous FRAP, FLIM and FAIM for measurements of protein mobility and interaction in living cells.Biomedical optics express · 2015Article
- Newtonian cell interactions shape natural killer cell education.Immunological reviews · 2015Review
- The limits of chemosensation vary across dimensions.Nature communications · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the supramolecular organization of killer Ig-like receptor (KIR) phosphorylation using a technique applicable to imaging phosphorylation of any green fluorescent protein-tagged receptor at an intercellular contact or immune synapse. Specifically, we use fluorescence lifetime imaging (FLIM) to report Förster resonance energy transfer (FRET) between GFP-tagged KIR2DL1 and a Cy3-tagged generic anti-phosphotyrosine monoclonal antibody. Visualization of KIR phosphorylation in natural killer (NK) cells contacting target cells expressing cognate major histocompatibility complex class I proteins revealed that inhibitory signaling is spatially restricted to the immune synapse. This explains how NK cells respond appropriately when simultaneously surveying susceptible and resistant target cells. More surprising, phosphorylated KIR was confined to microclusters within the aggregate of KIR, contrary to an expected homogeneous distribution of KIR signaling across the immune synapse. Also, yellow fluorescent protein-tagged Lck, a kinase important for KIR phosphorylation, accumulated in a multifocal distribution at inhibitory synapses. Spatial confinement of receptor phosphorylation within the immune synapse may be critical to how activating and inhibitory signals are integrated in NK cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.