Evidence map›Paper›PMID 16799077›Full record

ArticleAmerican journal of respiratory and critical care medicine2006

Fibroblast foci are not discrete sites of lung injury or repair: the fibroblast reticulum.

Carlyne D Cool, Steve D Groshong, Pradeep R Rai, Peter M Henson, J Scott Stewart, Kevin K Brown

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of respiratory and critical care medicine, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.

0numbers the graph read from it
0cells of the map it votes in
93citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

93 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Review
  6. Article
  7. Review
  8. Article
  9. Review
  10. Review
  11. Article
  12. Review
  13. Review
  14. Article
  15. Biomechanical Force and Cellular Stiffness in Lung Fibrosis.The American journal of pathology · 2022
    Review
  16. Article
  17. Review
  18. Stereology as the 3D tool to quantitate lung architecture.Histochemistry and cell biology · 2021
    Review
  19. Article
  20. Review

33 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Carlyne D CoolDepartments of Medicine and Pathology, University of Colorado Health Sciences Center; and Interstitial Lung Disease Program, National Jewish Medical and Research Center, Denver, Colorado 80206, USA.
Steve D Groshong
Pradeep R Rai
Peter M Henson
J Scott Stewart
Kevin K Brown

Funding

SCOR: Pathobiology of Fibrotic Lung DiseaseP50HL067671 · NHLBI · NATIONAL JEWISH HEALTH · PI MASON, ROBERT JAMES · 2002 to 2005
$7.2M
SCOR: Pathobiology of Fibrotic Lung DiseaseP01HL067671 · NHLBI · NATIONAL JEWISH HEALTH · PI HENSON, PETER M · 2001 to 2001
$1.7M
NHLBI NIH HHS HL67671
6 · The paper itself

Abstract

backgroundUsual interstitial pneumonia (UIP), the pathologic correlate of idiopathic pulmonary fibrosis, contains characteristic discrete areas of fibroblasts, myofibroblasts, and newly formed collagen, termed "fibroblast foci." These lesions are argued to represent isolated sites of recurrent acute lung injury and suggested to be the mechanism of disease progression. We hypothesized that, rather than isolated, these lesions are part of an organized neoplasm.

methodsMorphometric analysis of pentachrome-stained histologic sections of UIP was performed. Using point-counting technique on serial sections, fibroblast foci, arteries, and macrophage clusters were identified and we determined their individual "connectiveness" by estimating the Euler number. Two-dimensional micrographs were collated into a three-dimensional array from which a visual three-dimensional reconstruction could be constructed. Clonality analysis was performed using human androgen receptor gene methylation assay.

resultsBlood vessels show significant connectivity with a Euler number of 2, whereas macrophage clusters exhibited no connectivity. The fibroblast foci showed a high level of interconnection with Euler numbers ranging from 19 to 39. The computer generated three-dimensional models provide a visual confirmation of this connectiveness. Human androgen receptor gene methylation assay analysis of the foci showed balanced methylation consistent with polyclonality.

conclusionsThe fibroblast foci of UIP are the leading edge of a complex reticulum that is highly interconnected and extends from the pleura into the underlying parenchyma. It is a reactive, rather than a malignant, process.

Indexed as

Disease ProgressionFemaleFibroblastsHumansImage Processing, Computer-AssistedIn Vitro TechniquesLungLung Diseases, InterstitialPhotomicrographyProspective StudiesRespiratory Distress Syndrome

Identifiers

PMID16799077
PMCPMC2648056

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.