ArticleAmerican journal of respiratory cell and molecular biology2006
Induction of the plasminogen activator system by mechanical stimulation of human bronchial epithelial cells.
Article in American journal of respiratory cell and molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 81 citations in OpenAlex.
- Pooled it
- A targeted antioxidant nanomedicine regulates mitochondrial ROS and antiviral immunity in rhinovirus-infected human bronchial epithelial cells.Drug delivery and translational research · 2026Article
- Mechanically induced dysregulation of calcium homeostasis: a molecular linchpin in asthmatic pathogenesis.European journal of medical research · 2025Review
- TRPV2 modulates mechanically Induced ATP Release from Human bronchial epithelial cells.Respiratory research · 2024Article
- Immunologic aspects of asthma: from molecular mechanisms to disease pathophysiology and clinical translation.Frontiers in immunology · 2024Review
- Elevated PLAUR is observed in the airway epithelium of asthma patients and blocking improves barrier integrity.Clinical and translational allergy · 2023Article
- Rhinovirus infection induces secretion of endothelin-1 from airway epithelial cells in both in vitro and in vivo models.Respiratory research · 2023Article
- Mechanical forces suppress antiviral innate immune responses from asthmatic airway epithelial cells following rhinovirus infection.American journal of physiology. Lung cellular and molecular physiology · 2023Article
- Endothelial Dysfunction in Neurodegenerative Diseases.International journal of molecular sciences · 2023Review
- Airway mechanical compression: its role in asthma pathogenesis and progression.European respiratory review : an official journal of the European Respiratory Society · 2020Review
- Irradiation Induces Epithelial Cell Unjamming.Frontiers in cell and developmental biology · 2020Article
- Airway Epithelial Cell Immunity Is Delayed During Rhinovirus Infection in Asthma and COPD.Frontiers in immunology · 2020Article
- Sex-specific airway hyperreactivity and sex-specific transcriptome remodeling in neonatal piglets challenged with intra-airway acid.American journal of physiology. Lung cellular and molecular physiology · 2019Article
- Matrix metalloproteinase-9 deficiency protects mice from severe influenza A viral infection.JCI insight · 2018Article
- Asthma.Nature reviews. Disease primers · 2015Review
- Putting the Squeeze on Airway Epithelia.Physiology (Bethesda, Md.) · 2015Review
- Cross-talk between human mast cells and bronchial epithelial cells in plasminogen activator inhibitor-1 production via transforming growth factor-β1.American journal of respiratory cell and molecular biology · 2015Article
- Airway remodelling in asthma: role for mechanical forces.Asia Pacific allergy · 2014Review
- Plasminogen-stimulated airway smooth muscle cell proliferation is mediated by urokinase and annexin A2, involving plasmin-activated cell signalling.British journal of pharmacology · 2013Article
- Urokinase receptor orchestrates the plasminogen system in airway epithelial cell function.Lung · 2013Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Mechanical stimulation of the airway epithelium, as would occur during bronchoconstriction, is a potent stimulus and can activate profibrotic pathways. We used DNA microarray technology to examine gene expression in compressed normal human bronchial epithelial cells (NHBE). Compressive stress applied continuously over an 8-h period to NHBE cells led to the upregulation of several families of genes, including a family of plasminogen-related genes that were previously not known to be regulated in this system. Real-time PCR demonstrated a peak increase in gene expression of 8.0-fold for urokinase plasminogen activator (uPA), 16.2-fold for urokinase plasminogen activator receptor (uPAR), 4.2-fold for plasminogen activator inhibitor-1 (PAI-1), and 3.9-fold for tissue plasminogen activator (tPA). Compressive stress also increased uPA protein levels in the cell lysates (112.0 versus 82.0 ng/ml, P = 0.0004), and increased uPA (4.7 versus 3.3 ng/ml, P = 0.02), uPAR (1.3 versus 0.86 ng/ml, P = 0.007), and PAI-1 (50 versus 36 ng/ml, P = 0.006) protein levels in cell culture media. Functional studies demonstrated increased urokinase-dependent plasmin generation in compression-stimulated cells (0.0090 versus 0.0033 OD/min, P = 0.03). In addition, compression led to increased activation of matrix metalloproteinase (MMP)-9 and MMP-2 in a urokinase-dependent manner. In postmortem human lung tissue, we observed an increase in epithelial uPA and uPAR immunostaining in the airways of two patients who died in status asthmaticus compared with minimal immunoreactivity noted in airways from seven lung donors without asthma. Together these observations suggest an integrated response of airway epithelial cells to mechanical stimulation, acting through the plasminogen-activating system to modify the airway microenvironment.
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What OpenQuestion holds
Registered trials
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