ArticleMethods (San Diego, Calif.)2006
Application of photobleaching for measuring diffusion of prion proteins in cytosol of yeast cells.
Article in Methods (San Diego, Calif.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- Stress granules and protein aggregates reveal intracellular resource competition.bioRxiv : the preprint server for biology · 2025Article
- An adenine model of inborn metabolism errors alters TDP-43 aggregation and reduces its toxicity in yeast revealing insights into protein misfolding diseases.Microbial cell (Graz, Austria) · 2025Article
- Identification of protein aggregates in the aging vertebrate brain with prion-like and phase-separation properties.Cell reports · 2024Article
- Identification and Characterization of Sterol Acyltransferases Responsible for Steryl Ester Biosynthesis in Tomato.Frontiers in plant science · 2018Article
- Heat shock protein 104 (Hsp104)-mediated curing of [The Journal of biological chemistry · 2017Article
- Tomato UDP-Glucose Sterol Glycosyltransferases: A Family of Developmental and Stress Regulated Genes that Encode Cytosolic and Membrane-Associated Forms of the Enzyme.Frontiers in plant science · 2017Article
- Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase.Plant biotechnology journal · 2016Article
- Dissecting protein reaction dynamics in living cells by fluorescence recovery after photobleaching.Nature protocols · 2015Article
- Visualization of pinholin lesions in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Hsp70 targets Hsp100 chaperones to substrates for protein disaggregation and prion fragmentation.The Journal of cell biology · 2012Article
- Localization of HET-S to the cell periphery, not to [Het-s] aggregates, is associated with [Het-s]-HET-S toxicity.Molecular and cellular biology · 2012Article
- Dynamic SpoIIIE assembly mediates septal membrane fission during Bacillus subtilis sporulation.Genes & development · 2010Article
- Application of GFP-labeling to study prions in yeast.Protein and peptide letters · 2009Review
- Light-guided surface engineering for biomedical applications.Bioconjugate chemistry · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Measurement of fluorescence recovery after photobleaching (FRAP) is a non-invasive technique for studying protein dynamics in real time in living cells. FRAP studies are carried out on proteins tagged with green fluorescent protein (GFP) or one of its spectral variants. Illumination with high intensity laser light irreversibly bleaches the GFP fluorescence but has no effect on protein function. By photobleaching a limited region of the cytoplasm, the rate of fluorescence recovery provides a measure of the rate of protein diffusion. A detailed description of the FRAP technique is given, including its application to measuring the mobility of GFP-tagged Sup35p in [psi(-)] and [PSI(+)] cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.