ArticleNeurochemical research2006
Ascorbate transport and recycling by SH-SY5Y neuroblastoma cells: response to glutamate toxicity.
Article in Neurochemical research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- Vitamin C Status and Cognitive Function: A Systematic Review.Nutrients · 2017Pooled it
- Review
- On the effect of vitamin C intake on human health: How to (mis)interprete the clinical evidence.Redox biology · 2020Review
- Plasma Vitamin C Concentrations and Cognitive Function: A Cross-Sectional Study.Frontiers in aging neuroscience · 2019Article
- Dopamine Promotes Ascorbate Release from Retinal Neurons: Role of DMolecular neurobiology · 2018Article
- Sodium-dependent vitamin C transporter-2 mediates vitamin C transport at the cortical nerve terminal.Journal of neuroscience research · 2015Article
- Vitamin C neuroprotection against dose-dependent glutamate-induced neurodegeneration in the postnatal brain.Neurochemical research · 2015Article
- Ascorbic acid transport in brain microvascular pericytes.Biochemical and biophysical research communications · 2015Article
- Curcumin Protects Neurons from Glutamate-Induced Excitotoxicity by Membrane Anchored AKAP79-PKA Interaction Network.Evidence-based complementary and alternative medicine : eCAM · 2015Article
- A rapid and specific microplate assay for the determination of intra- and extracellular ascorbate in cultured cells.Journal of visualized experiments : JoVE · 2014Article
- Mitochondrial uncoupler carbonyl cyanide M-chlorophenylhydrazone induces the multimer assembly and activity of repair enzyme protein L-isoaspartyl methyltransferase.Journal of molecular neuroscience : MN · 2013Article
- Trapping of a cross-link formed by a major purine adduct of a metabolite of the carcinogen N-nitrosomorpholine by inorganic and biological reductants.Chemical research in toxicology · 2013Article
- Dynamic expression of the sodium-vitamin C co-transporters, SVCT1 and SVCT2, during perinatal kidney development.Histochemistry and cell biology · 2013Article
- Ascorbic acid efficiently enhances neuronal synthesis of norepinephrine from dopamine.Brain research bulletin · 2013Article
- Ascorbic acid: chemistry, biology and the treatment of cancer.Biochimica et biophysica acta · 2012Review
- Mechanisms of ascorbic acid stimulation of norepinephrine synthesis in neuronal cells.Biochemical and biophysical research communications · 2012Article
- The effect of monosodium glutamate on the cerebellar cortex of male albino rats and the protective role of vitamin C (histological and immunohistochemical study).Journal of molecular histology · 2012Article
- Urate and its transgenic depletion modulate neuronal vulnerability in a cellular model of Parkinson's disease.PloS one · 2012Article
- Vitamin C transport and its role in the central nervous system.Sub-cellular biochemistry · 2012Review
- Vitamin C function in the brain: vital role of the ascorbate transporter SVCT2.Free radical biology & medicine · 2009Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Neurons maintain relatively high intracellular concentrations of vitamin C, or ascorbic acid. In this work we studied the mechanisms by which neuronal cells in culture transport and maintain ascorbate, as well as how this system responds to oxidant stress induced by glutamate. Cultured SH-SY5Y neuroblastoma cells took up ascorbate, achieving steady-state intracellular concentrations of 6 mM and higher at extracellular concentrations of 200 microM and greater. This gradient was generated by relatively high affinity sodium-dependent ascorbate transport (Km of 113 microM). Ascorbate was also recycled from dehydroascorbate, the reduction of which was dependent on GSH, but not on D-glucose. Glutamate in concentrations up to 2 mM caused an acute concentration-dependent efflux of ascorbate from the cells, which was prevented by the anion channel blocker 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid. Intracellular ascorbate did not affect radiolabeled glutamate uptake, showing absence of heteroexchange.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.