ArticleVirus genes2006
Optimization of codon usage of poxvirus genes allows for improved transient expression in mammalian cells.
Article in Virus genes, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Discovery and investigation of the truncation of the (GGGGS)n linker and its effect on the productivity of bispecific antibodies expressed in mammalian cells.Bioprocess and biosystems engineering · 2025Article
- Novel strategies for development of hemorrhagic fever arenavirus live-attenuated vaccines.Expert review of vaccines · 2016Review
- Exploiting tRNAs to Boost Virulence.Life (Basel, Switzerland) · 2016Review
- Functional Comparison of Molluscum Contagiosum Virus vFLIP MC159 with Murine Cytomegalovirus M36/vICA and M45/vIRA Proteins.Journal of virology · 2015Article
- Genomic analysis of codon usage shows influence of mutation pressure, natural selection, and host features on Marburg virus evolution.BMC evolutionary biology · 2015Article
- Article
- Reply to "codon usage frequency of RNA virus genomes from high-temperature acidic-environment metagenomes".Journal of virology · 2013Article
- Genus specific evolution of codon usage and nucleotide compositional traits of poxviruses.Virus genes · 2011Article
- Autonomous bioluminescent expression of the bacterial luciferase gene cassette (lux) in a mammalian cell line.PloS one · 2010Article
- The impact of intragenic CpG content on gene expression.Nucleic acids research · 2010Article
- A replication-incompetent Rift Valley fever vaccine: chimeric virus-like particles protect mice and rats against lethal challenge.Virology · 2010Article
- Codon-optimized fluorescent proteins designed for expression in low-GC gram-positive bacteria.Applied and environmental microbiology · 2009Article
- Engineering the vaccinia virus L1 protein for increased neutralizing antibody response after DNA immunization.Virology journal · 2009Article
- Viral adaptation to host: a proteome-based analysis of codon usage and amino acid preferences.Molecular systems biology · 2009Article
- Ectromelia virus encodes a novel family of F-box proteins that interact with the SCF complex.Journal of virology · 2008Article
- Expression of frog virus 3 genes is impaired in mammalian cell lines.Virology journal · 2008Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transient expression of viral genes from certain poxviruses in uninfected mammalian cells can sometimes be unexpectedly inefficient. The reasons for poor expression levels can be due to a number of features of the gene cassette, such as cryptic splice sites, polymerase II termination sequences or motifs that lead to mRNA instability. Here we suggest that in some cases the problem of low protein expression in transfected mammalian cells may be due to inefficient codon usage. We have observed that for many poxvirus genes from the yatapoxvirus genus this deficiency can be overcome by synthesis of the gene with codon sequences optimized for expression in primate cells. This led us to examine colon usage across 2-dozen sequenced members of the Poxviridae. We conclude that codon usage is surprisingly divergent across the different Poxviridae genera but is much more conserved within a single genus. Thus, Poxviridae genera can be divided into distinct groups based on their observed codon bias. When viewed in this context, successful transient expression of transfected poxvirus genes in uninfected mammalian cells can be more accurately predicted based on codon bias. As a corollary, for specific poxvirus genes with less favorable codon usage, codon optimization can result in profoundly increased transient expression levels following transfection of uninfected mammalian cell lines.
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