ArticleInfection and immunity2006
Search for Bacillus anthracis potential vaccine candidates by a functional genomic-serologic screen.
Article in Infection and immunity, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 63 citations in OpenAlex.
- Peptidoglycan NlpC/P60 peptidases in bacterial physiology and host interactions.Cell chemical biology · 2023Review
- Development of ELISA based on Bacillus anthracis capsule biosynthesis protein CapA for naturally acquired antibodies against anthrax.PloS one · 2021Article
- TheMicroorganisms · 2020Review
- A new structural class of bacterial thioester domains reveals a slipknot topology.Protein science : a publication of the Protein Society · 2018Article
- Progress toward the Development of a NEAT Protein Vaccine for Anthrax Disease.Infection and immunity · 2016Article
- Changes in Bacillus anthracis CodY regulation under host-specific environmental factor deprived conditions.BMC genomics · 2016Article
- Next-Generation Bacillus anthracis Live Attenuated Spore Vaccine Based on the htrA(-) (High Temperature Requirement A) Sterne Strain.Scientific reports · 2016Article
- An update on the transport and metabolism of iron in Listeria monocytogenes: the role of proteins involved in pathogenicity.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2015Review
- Immunization of mice with formalin-inactivated spores from avirulent Bacillus cereus strains provides significant protection from challenge with Bacillus anthracis Ames.Clinical and vaccine immunology : CVI · 2013Article
- Identification of the immunogenic spore and vegetative proteins of Bacillus anthracis vaccine strain A16R.PloS one · 2013Article
- Hal Is a Bacillus anthracis heme acquisition protein.Journal of bacteriology · 2012Article
- Differential contribution of Bacillus anthracis toxins to pathogenicity in two animal models.Infection and immunity · 2012Article
- Investigating the genome diversity of B. cereus and evolutionary aspects of B. anthracis emergence.Genomics · 2011Article
- Plasmodium immunomics.International journal for parasitology · 2011Review
- Immunospecific responses to bacterial elongation factor Tu during Burkholderia infection and immunization.PloS one · 2010Article
- Genetic analysis of petrobactin transport in Bacillus anthracis.Molecular microbiology · 2010Article
- The adenylyl cyclase activity of anthrax edema factor.Molecular aspects of medicine · 2009Review
- Penetration of the blood-brain barrier by Bacillus anthracis requires the pXO1-encoded BslA protein.Journal of bacteriology · 2009Article
- Novel and unique diagnostic biomarkers for Bacillus anthracis infection.Applied and environmental microbiology · 2009Article
- Candidate antigens for Q fever serodiagnosis revealed by immunoscreening of a Coxiella burnetii protein microarray.Clinical and vaccine immunology : CVI · 2008Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus anthracis proteins that possess antigenic properties and are able to evoke an immune response were identified by a reductive genomic-serologic screen of a set of in silico-preselected open reading frames (ORFs). The screen included in vitro expression of the selected ORFs by coupled transcription and translation of linear PCR-generated DNA fragments, followed by immunoprecipitation with antisera from B. anthracis-infected animals. Of the 197 selected ORFs, 161 were chromosomal and 36 were on plasmids pXO1 and pXO2, and 138 of the 197 ORFs had putative functional annotations (known ORFs) and 59 had no assigned functions (unknown ORFs). A total of 129 of the known ORFs (93%) could be expressed, whereas only 38 (64%) of the unknown ORFs were successfully expressed. All 167 expressed polypeptides were subjected to immunoprecipitation with the anti-B. anthracis antisera, which revealed 52 seroreactive immunogens, only 1 of which was encoded by an unknown ORF. The high percentage of seroreactive ORFs among the functionally annotated ORFs (37%; 51/129) attests to the predictive value of the bioinformatic strategy used for vaccine candidate selection. Furthermore, the experimental findings suggest that surface-anchored proteins and adhesins or transporters, such as cell wall hydrolases, proteins involved in iron acquisition, and amino acid and oligopeptide transporters, have great potential to be immunogenic. Most of the seroreactive ORFs that were tested as DNA vaccines indeed appeared to induce a humoral response in mice. We list more than 30 novel B. anthracis immunoreactive virulence-related proteins which could be useful in diagnosis, pathogenesis studies, and future anthrax vaccine development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.