ArticleMolecular biology of the cell2006
The novel F-box protein Mfb1p regulates mitochondrial connectivity and exhibits asymmetric localization in yeast.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Comparative analysis of Cdc48-dependent proteolysis at the ER, mitochondria and chloroplasts.Nature communications · 2026Review
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- Proteolysis of adaptor protein Mmr1 during budding is necessary for mitochondrial homeostasis in Saccharomyces cerevisiae.Nature communications · 2022Article
- A role for cell polarity in lifespan and mitochondrial quality control in the budding yeastiScience · 2022Article
- Solid-phase inclusion as a mechanism for regulating unfolded proteins in the mitochondrial matrix.Science advances · 2020Article
- Asymmetric inheritance of spindle microtubule-organizing centres preserves replicative lifespan.Nature cell biology · 2019Article
- Mitochondrial anchors: Positioning mitochondria and more.Biochemical and biophysical research communications · 2018Review
- Mitochondrial Tethers and Their Impact on Lifespan in Budding Yeast.Frontiers in cell and developmental biology · 2017Review
- A role for Mfb1p in region-specific anchorage of high-functioning mitochondria and lifespan in Saccharomyces cerevisiae.Nature communications · 2016Article
- Glycosylation of Skp1 promotes formation of Skp1-cullin-1-F-box protein complexes in dictyostelium.Molecular & cellular proteomics : MCP · 2015Article
- Dynamic survey of mitochondria by ubiquitin.EMBO reports · 2014Review
- Review
- Indispensable role for the eukaryotic-like ankyrin domains of the ankyrin B effector of Legionella pneumophila within macrophages and amoebae.Infection and immunity · 2010Article
- Molecular mimicry by an F-box effector of Legionella pneumophila hijacks a conserved polyubiquitination machinery within macrophages and protozoa.PLoS pathogens · 2009Article
- Lessons from fungal F-box proteins.Eukaryotic cell · 2009Review
- The novel tail-anchored membrane protein Mff controls mitochondrial and peroxisomal fission in mammalian cells.Molecular biology of the cell · 2008Article
- Tetratricopeptide repeat proteins Tom70 and Tom71 mediate yeast mitochondrial morphogenesis.EMBO reports · 2008Article
- Characterization of the regulatory and expression context of an alternative oxidase gene provides insights into cyanide-insensitive respiration during growth and development.Plant physiology · 2007Article
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Authors and funding
6 authors at 3 institutions in 1 country.
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Abstract
Although it is clear that mitochondrial morphogenesis is a complex process involving multiple proteins in eukaryotic cells, little is known about regulatory molecules that modulate mitochondrial network formation. Here, we report the identification of a new yeast mitochondrial morphology gene called MFB1 (YDR219C). MFB1 encodes an F-box protein family member, many of which function in Skp1-Cdc53/Cullin-F-box protein (SCF) ubiquitin ligase complexes. F-box proteins also act in non-SCF complexes whose functions are not well understood. Although cells lacking Mfb1p contain abnormally short mitochondrial tubules, Mfb1p is not essential for known pathways that determine mitochondrial morphology and dynamics. Mfb1p is peripherally associated with the mitochondrial surface. Coimmunoprecipitation assays reveal that Mfb1p interacts with Skp1p in an F-box-dependent manner. However, Mfb1p does not coimmunoprecipitate with Cdc53p. The F-box motif is not essential for Mfb1p-mediated mitochondrial network formation. These observations suggest that Mfb1p acts in a complex lacking Cdc53p required for mitochondrial morphogenesis. During budding, Mfb1p asymmetrically localizes to mother cell mitochondria. By contrast, Skp1p accumulates in the daughter cell cytoplasm. Mfb1p mother cell-specific asymmetry depends on the F-box motif, suggesting that Skp1p down-regulates Mfb1p mitochondrial association in buds. We propose that Mfb1p operates in a novel pathway regulating mitochondrial tubular connectivity.
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