ArticleMolecular biology of the cell2006
Guanylyl cyclase protein and cGMP product independently control front and back of chemotaxing Dictyostelium cells.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 49 citations in OpenAlex.
- The Proteome ofProteomes · 2026Article
- Diverse Roles of the Multiple Phosphodiesterases in the Regulation of Cyclic Nucleotide Signaling inCells · 2025Review
- Forty-five years of cGMP research inMolecular biology of the cell · 2021Review
- Short- and long-term memory of moving amoeboid cells.PloS one · 2021Article
- Pumilio-dependent localization of mRNAs at the cell front coordinates multiple pathways required for chemotaxis.Nature communications · 2017Article
- Article
- Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes.Cellular and molecular life sciences : CMLS · 2014Review
- Dissecting the Molecular Mechanisms of Electrotactic Effects.Advances in wound care · 2014Review
- Dictyostelium Ric8 is a nonreceptor guanine exchange factor for heterotrimeric G proteins and is important for development and chemotaxis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Daydreamer, a Ras effector and GSK-3 substrate, is important for directional sensing and cell motility.Molecular biology of the cell · 2013Article
- Multiple regulatory mechanisms for the Dictyostelium Roco protein GbpC.The Journal of biological chemistry · 2012Article
- Article
- Dictyostelium chemotaxis: essential Ras activation and accessory signalling pathways for amplification.EMBO reports · 2011Article
- Signaling mechanisms for chemotaxis.Development, growth & differentiation · 2011Review
- A stochastic model for chemotaxis based on the ordered extension of pseudopods.Biophysical journal · 2010Article
- A model for a correlated random walk based on the ordered extension of pseudopodia.PLoS computational biology · 2010Article
- Navigation of chemotactic cells by parallel signaling to pseudopod persistence and orientation.PloS one · 2009Article
- The local cell curvature guides pseudopodia towards chemoattractants.HFSP journal · 2009Article
- Switching direction in electric-signal-induced cell migration by cyclic guanosine monophosphate and phosphatidylinositol signaling.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The ordered extension of pseudopodia by amoeboid cells in the absence of external cues.PloS one · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chemotaxis of amoeboid cells is driven by actin filaments in leading pseudopodia and actin-myosin filaments in the back and at the side of the cell to suppress pseudopodia. In Dictyostelium, cGMP plays an important role during chemotaxis and is produced predominantly by a soluble guanylyl cyclase (sGC). The sGC protein is enriched in extending pseudopodia at the leading edge of the cell during chemotaxis. We show here that the sGC protein and the cGMP product have different functions during chemotaxis, using two mutants that lose either catalytic activity (sGCDelta cat) or localization to the leading edge (sGCDeltaN). Cells expressing sGCDeltaN exhibit excellent cGMP formation and myosin localization in the back of the cell, but they exhibit poor orientation at the leading edge. Cells expressing the catalytically dead sGCDelta cat mutant show poor myosin localization at the back, but excellent localization of the sGC protein at the leading edge, where it enhances the probability that a new pseudopod is made in proximity to previous pseudopodia, resulting in a decrease of the degree of turning. Thus cGMP suppresses pseudopod formation in the back of the cell, whereas the sGC protein refines pseudopod formation at the leading edge.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.