ArticleJournal of bacteriology2006
Protein-protein interactions among Helicobacter pylori cag proteins.
Article in Journal of bacteriology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 74 citations in OpenAlex.
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- Roles of the components of theFuture microbiology · 2024Review
- Role of the CagY antenna projection inInfection and immunity · 2023Article
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- Bacterial Energetic Requirements for Helicobacter pylori Cag Type IV Secretion System-Dependent Alterations in Gastric Epithelial Cells.Infection and immunity · 2020Article
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- Acid-regulated gene expression of Helicobacter pylori: Insight into acid protection and gastric colonization.Helicobacter · 2018Article
- Prevalence of CagA and antimicrobial sensitivity ofInfectious agents and cancer · 2018Article
- Analyzing the role of CagV, a VirB8 homolog of the type IV secretion system ofFEBS open bio · 2017Article
- The Helicobacter pylori Cag Pathogenicity Island Protein Cag1 is Associated with the Function of T4SS.Current microbiology · 2016Article
- Article
- Genes required for assembly of pili associated with the Helicobacter pylori cag type IV secretion system.Infection and immunity · 2014Article
- At the Bench: Helicobacter pylori, dysregulated host responses, DNA damage, and gastric cancer.Journal of leukocyte biology · 2014Review
- Analysis of surface-exposed outer membrane proteins in Helicobacter pylori.Journal of bacteriology · 2014Article
- In depth analysis of the Helicobacter pylori cag pathogenicity island transcriptional responses.PloS one · 2014Article
- Control of gene expression in Helicobacter pylori using the Tet repressor.Journal of microbiological methods · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Many Helicobacter pylori isolates contain a 40-kb region of chromosomal DNA known as the cag pathogenicity island (PAI). The risk for development of gastric cancer or peptic ulcer disease is higher among humans infected with cag PAI-positive H. pylori strains than among those infected with cag PAI-negative strains. The cag PAI encodes a type IV secretion system that translocates CagA into gastric epithelial cells. To identify Cag proteins that are expressed by H. pylori during growth in vitro, we compared the proteomes of a wild-type H. pylori strain and an isogenic cag PAI deletion mutant using two-dimensional difference gel electrophoresis (2D-DIGE) in multiple pH ranges. Seven Cag proteins were identified by this approach. We then used a yeast two-hybrid system to detect potential protein-protein interactions among 14 Cag proteins. One heterotypic interaction (CagY/7 with CagX/8) and two homotypic interactions (involving H. pylori VirB11/ATPase and Cag5) were similar to interactions previously reported to occur among homologous components of the Agrobacterium tumefaciens type IV secretion system. Other interactions involved Cag proteins that do not have known homologues in other bacterial species. Biochemical analysis confirmed selected interactions involving five of the proteins that were identified by 2D-DIGE. Protein-protein interactions among Cag proteins are likely to have an important role in the assembly of the H. pylori type IV secretion apparatus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.