ArticleAmerican journal of physiology. Renal physiology2006
Expression and distribution of HuR during ATP depletion and recovery in proximal tubule cells.
Article in American journal of physiology. Renal physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- RNA-binding proteins and their role in kidney disease.Nature reviews. Nephrology · 2022Review
- Purinergic signalling in the kidney in health and disease.Purinergic signalling · 2014Review
- Krüppel -like factor 8 is a stress-responsive transcription factor that regulates expression of HuR.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2014Article
- Adaptive and maladaptive expression of the mRNA regulatory protein HuR.World journal of biological chemistry · 2013Review
- Article
- HuR inhibits apoptosis by amplifying Akt signaling through a positive feedback loop.Journal of cellular physiology · 2013Article
- Article
- Concurrent binding and modifications of AUF1 and HuR mediate the pH-responsive stabilization of phosphoenolpyruvate carboxykinase mRNA in kidney cells.American journal of physiology. Renal physiology · 2012Article
- The H19 lincRNA is a developmental reservoir of miR-675 that suppresses growth and Igf1r.Nature cell biology · 2012Article
- Role of AUF1 and HuR in the pH-responsive stabilization of phosphoenolpyruvate carboxykinase mRNA in LLC-PK₁-F⁺ cells.American journal of physiology. Renal physiology · 2011Article
- Transcriptional control of human antigen R by bone morphogenetic protein.The Journal of biological chemistry · 2010Article
- Neuronal expression of splice variants of "glial" glutamate transporters in brains afflicted by Alzheimer's disease: unmasking an intrinsic neuronal property.Neurochemical research · 2009Article
- Expression of the RNA-stabilizing protein HuR in ischemia-reperfusion injury of rat kidney.American journal of physiology. Renal physiology · 2009Article
- ATP modulates PTEN subcellular localization in multiple cancer cell lines.Human molecular genetics · 2008Article
- Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Human antigen R (HuR) is a nucleocytoplasmic shuttling protein that binds to and stabilizes mRNAs containing adenine- and uridine-rich elements. Under normal growth conditions, the bulk of HuR is maintained in the nucleus, but under conditions of cell stress, HuR may become more prevalent in the cytosol, where it can stabilize mRNA and regulate gene expression. We have studied the behavior of HuR in LLC-PK1 proximal tubule cells subjected to ATP depletion and recovery. ATP depletion resulted in detectable net movement of HuR out of the nucleus, followed by net movement of HuR back into the nucleus on reversion to normal growth medium. In addition, HuR protein levels increased during energy depletion. This increase was inhibited by cycloheximide and was independent of HuR mRNA levels, since no change was noted in the quantity of HuR transcript. In contrast, recovery in normal growth medium resulted in increased HuR mRNA, while protein levels decreased to baseline. This suggested a mechanism by which previously injured cells maintained normal levels of HuR but were primed to rapidly translate increased amounts of protein on subsequent insults. Indeed, a second round of ATP depletion resulted in heightened HuR protein translation at a rate more rapid than during the first insult. Additionally, the second insult produced increased HuR levels in the cytoplasm while still maintaining high amounts in the nucleus, indicating that nuclear export may not be required on subsequent insults. These results suggest a role for HuR in protecting kidney epithelia from injury during ischemic stress.
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