ArticleProceedings of the National Academy of Sciences of the United States of America2006
Recruitment of mRNA cleavage/polyadenylation machinery by the yeast chromatin protein Sin1p/Spt2p.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- A novel gene, Le-Dd10, is involved in fruiting body formation of Lentinula edodes.Archives of microbiology · 2022Article
- SCRaMbLE generates evolved yeasts with increased alkali tolerance.Microbial cell factories · 2019Article
- Multiple transcription factors contribute to inter-chromosomal interaction in yeast.BMC systems biology · 2018Article
- A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.G3 (Bethesda, Md.) · 2012Article
- The spt5 C-terminal region recruits yeast 3' RNA cleavage factor I.Molecular and cellular biology · 2012Article
- Article
- RNase H and multiple RNA biogenesis factors cooperate to prevent RNA:DNA hybrids from generating genome instability.Molecular cell · 2011Article
- Transcription regulation by the noncoding RNA SRG1 requires Spt2-dependent chromatin deposition in the wake of RNA polymerase II.Molecular and cellular biology · 2011Article
- Spt2p defines a new transcription-dependent gross chromosomal rearrangement pathway.PLoS genetics · 2008Article
- Direct interactions between the Paf1 complex and a cleavage and polyadenylation factor are revealed by dissociation of Paf1 from RNA polymerase II.Eukaryotic cell · 2008Article
- Analysis of transcriptional activation at a distance in Saccharomyces cerevisiae.Molecular and cellular biology · 2007Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The yeast chromatin protein Sin1p/Spt2p has long been studied, but the understanding of its function has remained elusive. The protein has sequence similarity to HMG1, specifically binds crossing DNA structures, and serves as a negative transcriptional regulator of a small family of genes that are activated by the SWI/SNF chromatin-remodeling complex. Recently, it has been implicated in maintaining the integrity of chromatin during transcription elongation. Here we present experiments whose results indicate that Sin1p/Spt2 is required for, and is directly involved in, the efficient recruitment of the mRNA cleavage/polyadenylation complex. This conclusion is based on the following findings: Sin1p/Spt2 frequently binds specifically downstream of many ORFs but almost always upstream of the first polyadenylation site. It directly interacts with Fir1p, a component of the cleavage/polyadenylation complex. Disruption of Sin1p/Spt2p results in foreshortened poly(A) tracts on mRNA. It is synthetically lethal with Cdc73p, which is involved in the recruitment of the complex. This report shows that a chromatin component is involved in 3' end processing of RNA.
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