ArticleJournal of molecular evolution2006
Molecular cloning of disintegrin-like transcript BA-5A from a Bitis arietans venom gland cDNA library: a putative intermediate in the evolution of the long-chain disintegrin bitistatin.
Article in Journal of molecular evolution, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 63 citations in OpenAlex.
- Biochemical characterisation and substrate-specific proteolytic diversity of venom metalloproteinases in African puff adders.Toxicon: X · 2026Article
- Clinical features of puff adder envenoming: case series of Bitis arietans snakebites in Kenya and a scoping review of the literature.PLoS neglected tropical diseases · 2025Article
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- Inhibition of bacterial biofilms by the snake venom proteome.Biotechnology reports (Amsterdam, Netherlands) · 2023Article
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- Comparison of adjuvant emulsions for their safety and ability to enhance the antibody response in horses immunized with African snake venoms.Vaccine: X · 2022Article
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- Intrageneric cross-reactivity of monospecific rabbit antisera against venoms of the medically most important Bitis spp. and Echis spp. African snakes.PLoS neglected tropical diseases · 2022Article
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- Terrestrial venomous animals, the envenomings they cause, and treatment perspectives in the Middle East and North Africa.PLoS neglected tropical diseases · 2021Review
- Old World Vipers-A Review about Snake Venom Proteomics of Viperinae and Their Variations.Toxins · 2021Review
- Structure-Function Relationship of the Disintegrin Family: Sequence Signature and Integrin Interaction.Frontiers in molecular biosciences · 2021Review
- Isolation and Functional Identification of an Antiplatelet RGD-Containing Disintegrin from Cerastes cerastes Venom.The protein journal · 2020Article
- Cost of Manufacturing for Recombinant Snakebite Antivenoms.Frontiers in bioengineering and biotechnology · 2020Article
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- Kn-Ba: a novel serine protease isolated fromThe journal of venomous animals and toxins including tropical diseases · 2018Article
- Third Generation Antivenomics: Pushing the Limits of the In Vitro Preclinical Assessment of Antivenoms.Toxins · 2017Article
- Unusual stability of messenger RNA in snake venom reveals gene expression dynamics of venom replenishment.PloS one · 2012Article
- Antivenomic assessment of the immunological reactivity of EchiTAb-Plus-ICP, an antivenom for the treatment of snakebite envenoming in sub-Saharan Africa.The American journal of tropical medicine and hygiene · 2010Article
- Loss of introns along the evolutionary diversification pathway of snake venom disintegrins evidenced by sequence analysis of genomic DNA from Macrovipera lebetina transmediterranea and Echis ocellatus.Journal of molecular evolution · 2007Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the cloning and sequence analysis of BA-5A from a venom gland cDNA library of the puff adder, Bitis arietans, that encodes a novel ECD-disintegrin-like domain. BA-5A is a unique PII disintegrin. It contains the 16 cysteine residues that are conserved in all known disintegrin-like domains of ADAM proteins and snake venom metalloproteinases but lacks the cysteine-rich domain. These features suggest that BA-5A may represent an intermediate in the evolutionary pathway of the long disintegrin bitistatin and that removal of the cysteine-rich domain and loss of the PIII-specific disulfide bond were separate events along the structural diversification pathway of disintegrins, the former predating the latter. The protein family composition of the Bitis arietans venom, as determined by combination of reversed-phase HPLC and proteomic analysis, was as follows: Zn(2+)-metalloproteinase (38.5%), serine proteinase (19.5%), disintegrin (17.8%), C-type lectin-like (13.2%), PLA(2) (4.3%), Kunitz-type inhibitor (4.1%), cystatin (1.7%), and unknown (0.9%). BA-5A could not be detected in the venom proteome of Bitis arietans. The occurrence of this very low-abundance (< 0.05%) or nonexpressed disintegrin transcript indicates a hitherto unrecognized structural diversity of this protein family. Whether BA-5A plays a physiological role or represents an orphan protein which could eventually evolve a role in the adaptation of snakes to changing ecological niches and prey habits deserves further investigation.
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