ArticleProstate cancer and prostatic diseases2006
Characterization of a method for profiling gene expression in cells recovered from intact human prostate tissue using RNA linear amplification.
Article in Prostate cancer and prostatic diseases, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Trial
- MEK4 function, genistein treatment, and invasion of human prostate cancer cells.Journal of the National Cancer Institute · 2009Trial
- Androgen receptor-independent function of FoxA1 in prostate cancer metastasis.Cancer research · 2013Article
- Identification of markers of prostate cancer progression using candidate gene expression.British journal of cancer · 2012Article
- Endoglin suppresses human prostate cancer metastasis.Clinical & experimental metastasis · 2011Article
- Concise syntheses of the abyssinones and discovery of new inhibitors of prostate cancer and MMP-2 expression.ACS medicinal chemistry letters · 2010Article
- Evaluation of methods for amplification of picogram amounts of total RNA for whole genome expression profiling.BMC genomics · 2009Article
- Terminal Continuation (TC) RNA amplification enables expression profiling using minute RNA input obtained from mouse brain.International journal of molecular sciences · 2008Article
- The methodology used to measure differential gene expression affects the outcome.Journal of biomolecular techniques : JBT · 2007Article
- Article
- MyD88 predicts chemoresistance to paclitaxel in epithelial ovarian cancer.The Yale journal of biology and medicine · 2006Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Coupling array technology to laser capture microdissection (LCM) has the potential to yield gene expression profiles of specific cell populations within tissue. However, remaining problems with linear amplification preclude accurate expression profiling when using the low nanogram amounts of RNA recovered after LCM of human tissue. We describe a novel robust method to reliably amplify RNA after LCM, allowing direct probing of 12K gene arrays. The fidelity of amplification was demonstrated by comparing the ability of amplified RNA (aRNA) versus that of native RNA to identify differentially expressed genes between two different cell lines, demonstrating a 99.3% concordance between observations. Array findings were validated by quantitative polymerase chain reaction analysis of a randomly selected subset of 32 genes. Using LCM to recover normal (N=5 subjects) or cancer (N=3) cell populations from intact human prostate tissue, three differentially expressed genes were identified. Independent investigators have previously identified differential expression of two of these three genes, hepsin and beta-microseminoprotein, in prostate cancer. Taken together, the current study demonstrates that accurate gene expression profiling can readily be performed on specific cell populations present within complex tissue. It also demonstrates that this approach efficiently identifies biologically relevant genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.