Evidence map›Paper›PMID 16784416›Full record

ArticleThe Biochemical journal2006

Physiological stimulation regulates the exocytic mode through calcium activation of protein kinase C in mouse chromaffin cells.

Tiberiu Fulop, Corey Smith

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Biochemical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 64 citations in OpenAlex.

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  6. CaProceedings of the National Academy of Sciences of the United States of America · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Tiberiu FulopDepartment of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH 44106, USA.
Corey Smith
Case Western Reserve University · US

Funding

CLEVELAND TRAINING PROGRAM IN CARDIOVASCULAR RESEARCHT32HL007653 · NHLBI · CASE WESTERN RESERVE UNIVERSITY · PI DUBYAK, GEORGE R · 1988 to 2008
$3.5M
Mechanisms of the adrenal medulla stress response.R01NS052123 · NINDS · CASE WESTERN RESERVE UNIVERSITY · PI SMITH, COREY B · 2005 to 2008
$1.2M
NHLBI NIH HHS T32 HL007653NHLBI NIH HHS T32 HL 07653NINDS NIH HHS 1R01NS052123NINDS NIH HHS R01 NS052123
6 · The paper itself

Abstract

Adrenal medullary chromaffin cells release catecholamines and neuropeptides in an activity-dependent manner controlled by the sympathetic nervous system. Under basal sympathetic tone, catecholamines are preferentially secreted. During acute stress, increased sympathetic firing evokes release of both catecholamines as well as neuropeptides. Both signalling molecules are co-packaged in the same large dense core granules, thus release of neuropeptide transmitters must be regulated after granule fusion with the cell surface. Previous work has indicated this may be achieved through a size-exclusion mechanism whereby, under basal sympathetic firing, the catecholamines are selectively released through a restricted fusion pore, while less-soluble neuropeptides are left behind in the dense core. Only under the elevated firing experienced during the sympathetic stress response do the granules fully collapse to expel catecholamines and neuropeptides. However, mechanistic description and physiological regulation of this process remain to be determined. We employ electrochemical amperometry, fluid-phase dye uptake and electrophysiological capacitance noise analysis to probe the fusion intermediate in mouse chromaffin cells under physiological electrical stimulation. We show that basal firing rates result in the selective release of catecholamines through an Omega-form 'kiss and run' fusion event characterized by a narrow fusion pore. Increased firing raises calcium levels and activates protein kinase C, which then promotes fusion pore dilation until full granule collapse occurs. Our results demonstrate that the transition between 'kiss and run' and 'full collapse' exocytosis serves a vital physiological regulation in neuroendocrine chromaffin cells and help effect a proper acute stress response.

Indexed as

AnimalsCalciumCalcium SignalingCatecholaminesChromaffin CellsElectrochemistryEnzyme ActivationExocytosisMiceMice, Inbred C57BLNeuropeptidesProtein Kinase CCalciumCatecholaminesNeuropeptidesProtein Kinase C

Identifiers

PMID16784416
PMCPMC1570168
OpenAlexW1981471126

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.