ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2006
A sunflower BAC library suitable for PCR screening and physical mapping of targeted genomic regions.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
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- Development and Applications of Chromosome-Specific Cytogenetic BAC-FISH Probes inFrontiers in plant science · 2018Article
- Genotyping-by-sequencing targeting of a novel downy mildew resistance gene PlTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- High-throughput physical map anchoring via BAC-pool sequencing.BMC plant biology · 2015Article
- A colony multiplex quantitative PCR-Based 3S3DBC method and variations of it for screening DNA libraries.PloS one · 2015Article
- Evolutionary origins of a bioactive peptide buried within Preproalbumin.The Plant cell · 2014Article
- Global transcriptome analysis and identification of a CONSTANS-like gene family in the orchid Erycina pusilla.Planta · 2013Article
- Positional cloning of a candidate gene for resistance to the sunflower downy mildew, Plasmopara halstedii race 300.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Comparative analysis of a plant pseudoautosomal region (PAR) in Silene latifolia with the corresponding S. vulgaris autosome.BMC genomics · 2012Article
- Molecular mapping of the Pl(16) downy mildew resistance gene from HA-R4 to facilitate marker-assisted selection in sunflower.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Utilization of super BAC pools and Fluidigm access array platform for high-throughput BAC clone identification: proof of concept.Journal of biomedicine & biotechnology · 2012Article
- Genomic diversity in two related plant species with and without sex chromosomes--Silene latifolia and S. vulgaris.PloS one · 2012Article
- Advances in BAC-based physical mapping and map integration strategies in plants.Journal of biomedicine & biotechnology · 2012Review
- Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of Oncidium Gower Ramsey.BMC plant biology · 2011Article
- A Comparative BAC map for the gilthead sea bream (Sparus aurata L.).Journal of biomedicine & biotechnology · 2011Article
- Structure and evolution of Apetala3, a sex-linked gene in Silene latifolia.BMC plant biology · 2010Article
- Construction and characterization of two BAC libraries representing a deep-coverage of the genome of chicory (Cichorium intybus L., Asteraceae).BMC research notes · 2010Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A sunflower BAC library consisting of 147,456 clones with an average size of 118 kb has been constructed and characterized. It represents approximately 5x sunflower haploid genome equivalents. The BAC library has been arranged in pools and superpools of DNA allowing screening with various PCR-based markers. Each of the 32 superpools contains 4,608 clones and corresponds to a 36 matrix pools. Thus, the screening of the entire library could be accomplished in less than 80 PCR reactions including positive and negative controls. As a demonstration of the feasibility of the concept, a set of 24 SSR markers covering about 36 cM in the sunflower SSR map (Tang et al. in Theor Appl Genet 105:1124-1136, 2002) have been used to screen the BAC library. About 125 BAC clones have been identified and then organized in 23 contigs by HindIII digestion. The contigs are anchored on the SSR map and thus constitutes a first-generation physical map of this region. The utility of this BAC library as a genomic resource for physical mapping and map-based cloning in sunflower is discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.